ArticleInfection and immunity2003
Identification of a 3-deoxy-D-manno-octulosonic acid biosynthetic operon in Moraxella catarrhalis and analysis of a KdsA-deficient isogenic mutant.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Expression of the Oligopeptide Permease Operon of Moraxella catarrhalis Is Regulated by Temperature and Nutrient Availability.Infection and immunity · 2015Article
- Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis.Proteome science · 2014Article
- Characterization of a trifunctional glucosyltransferase essential for Moraxella catarrhalis lipooligosaccharide assembly.Glycobiology · 2013Article
- Use of the chinchilla model for nasopharyngeal colonization to study gene expression by Moraxella catarrhalis.Infection and immunity · 2012Article
- Ion mobility mass spectrometry for extracting spectra of N-glycans directly from incubation mixtures following glycan release: application to glycans from engineered glycoforms of intact, folded HIV gp120.Journal of the American Society for Mass Spectrometry · 2011Article
- Characterization of a Moraxella species that causes epistaxis in macaques.Veterinary microbiology · 2011Article
- Genome analysis of Moraxella catarrhalis strain BBH18, [corrected] a human respiratory tract pathogen.Journal of bacteriology · 2010Article
- Identification and characterization of a glycosyltransferase involved in Acinetobacter baumannii lipopolysaccharide core biosynthesis.Infection and immunity · 2010Article
- Use of Moraxella catarrhalis lipooligosaccharide mutants to identify specific oligosaccharide epitopes recognized by human serum antibodies.Infection and immunity · 2009Article
- Molecular aspects of Moraxella catarrhalis pathogenesis.Microbiology and molecular biology reviews : MMBR · 2009Review
- Comparative proteomic analysis of the Haemophilus ducreyi porin-deficient mutant 35000HP::P2AB.Journal of bacteriology · 2009Article
- Modular arrangement of allelic variants explains the divergence in Moraxella catarrhalis UspA protein function.Infection and immunity · 2008Article
- Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis.The FEBS journal · 2008Article
- Contribution of Moraxella catarrhalis type IV pili to nasopharyngeal colonization and biofilm formation.Infection and immunity · 2007Article
- Metabolic analysis of Moraxella catarrhalis and the effect of selected in vitro growth conditions on global gene expression.Infection and immunity · 2007Article
- Construction of a mutant and characterization of the role of the vaccine antigen P6 in outer membrane integrity of nontypeable Haemophilus influenzae.Infection and immunity · 2006Article
- Binding of vitronectin by the Moraxella catarrhalis UspA2 protein interferes with late stages of the complement cascade.Infection and immunity · 2006Article
- Biofilm formation by Moraxella catarrhalis in vitro: roles of the UspA1 adhesin and the Hag hemagglutinin.Infection and immunity · 2006Article
- Multiplex PCR assay that identifies the major lipooligosaccharide serotype expressed by Moraxella catarrhalis clinical isolates.Journal of clinical microbiology · 2005Article
- Moraxella catarrhalis bacterium without endotoxin, a potential vaccine candidate.Infection and immunity · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Lipooligosaccharide (LOS), a predominant surface-exposed component of the outer membrane, has been implicated as a virulence factor in the pathogenesis of Moraxella catarrhalis infections. However, the critical steps involved in the biosynthesis and assembly of M. catarrhalis LOS currently remain undefined. In this study, we used random transposon mutagenesis to identify a 3-deoxy-D-manno-octulosonic acid (KDO) biosynthetic operon in M. catarrhalis with the gene order pyrG-kdsA-eno. The lipid A-KDO molecule serves as the acceptor onto which a variety of glycosyl transferases sequentially add the core and branch oligosaccharide extensions for the LOS molecule. KdsA, the KDO-8-phosphate synthase, catalyzes the first step of KDO biosynthesis and is an essential enzyme in gram-negative enteric bacteria for maintenance of bacterial viability. We report the construction of an isogenic M. catarrhalis kdsA mutant in strain 7169 by allelic exchange. Our data indicate that an LOS molecule consisting only of lipid A and lacking KDO glycosylation is sufficient to sustain M. catarrhalis survival in vitro. In addition, comparative growth and susceptibility assays were performed to assess the sensitivity of 7169kdsA11 compared to that of the parental strain. The results of these studies demonstrate that the native LOS molecule is an important factor in maintaining the integrity of the outer membrane and suggest that LOS is a critical component involved in the ability of M. catarrhalis to resist the bactericidal activity of human sera.
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