ArticleInfection and immunity2003
Identification of a gene that affects the efficiency of host cell infection by Legionella pneumophila in a temperature-dependent fashion.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed.
- Evaluation of Genetic Diversity and Virulence Potential ofPathogens (Basel, Switzerland) · 2023Article
- Legionnaires' Disease Mortality in Guinea Pigs Involves the p45 Mobile Genomic Element.The Journal of infectious diseases · 2019Article
- Viewing Legionella pneumophila Pathogenesis through an Immunological Lens.Journal of molecular biology · 2019Review
- Legionella pneumophila p45 element influences host cell entry and sensitivity to sodium.PloS one · 2019Article
- Gene flow in environmental Legionella pneumophila leads to genetic and pathogenic heterogeneity within a Legionnaires' disease outbreak.Genome biology · 2014Article
- Use of Galleria mellonella as a model organism to study Legionella pneumophila infection.Journal of visualized experiments : JoVE · 2013Article
- Genome dynamics in Legionella: the basis of versatility and adaptation to intracellular replication.Cold Spring Harbor perspectives in medicine · 2013Review
- Virulence phenotypes of Legionella pneumophila associated with noncoding RNA lpr0035.Infection and immunity · 2012Article
- Random inducible controlled expression (RICE) for identification of mycobacterial virulence genes.Tuberculosis (Edinburgh, Scotland) · 2011Article
- Legionella pneumophila type II secretion dampens the cytokine response of infected macrophages and epithelia.Infection and immunity · 2011Article
- Legionella pneumophila strain 130b possesses a unique combination of type IV secretion systems and novel Dot/Icm secretion system effector proteins.Journal of bacteriology · 2010Article
- ArgR-regulated genes are derepressed in the Legionella-containing vacuole.Journal of bacteriology · 2010Article
- Molecular pathogenesis of infections caused by Legionella pneumophila.Clinical microbiology reviews · 2010Review
- Virulence factors encoded by Legionella longbeachae identified on the basis of the genome sequence analysis of clinical isolate D-4968.Journal of bacteriology · 2010Article
- The purified and recombinant Legionella pneumophila chaperonin alters mitochondrial trafficking and microfilament organization.Infection and immunity · 2009Article
- Many substrates and functions of type II secretion: lessons learned from Legionella pneumophila.Future microbiology · 2009Review
- Surface translocation by Legionella pneumophila: a form of sliding motility that is dependent upon type II protein secretion.Journal of bacteriology · 2009Article
- Use of gene dosage effects for a whole-genome screen to identify Mycobacterium marinum macrophage infection loci.Infection and immunity · 2008Article
- The secreted pyomelanin pigment of Legionella pneumophila confers ferric reductase activity.Infection and immunity · 2007Article
- Immunogenicity of Anaplasma marginale type IV secretion system proteins in a protective outer membrane vaccine.Infection and immunity · 2007Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The ability to infect host cells is critical for the survival and replication of intracellular pathogens in humans. We previously found that many genes involved in the ability of Legionella pneumophila to infect macrophages are not expressed efficiently under standard laboratory growth conditions. We have developed an approach using expression of L. pneumophila genes from an exogenous constitutive promoter on a low-copy-number vector that allows identification of genes involved in host cell infection. Through the use of this strategy, we found that expression of a gene, lvhB2, enhances the efficiency of L. pneumophila infection of mammalian cells. The putative protein encoded by lvhB2 has similarity to structural pilin subunits of type IV secretion systems. We confirmed that this gene plays a role in host cell infection by the construction of an in-frame deletion in the L. pneumophila lvhB2 gene and complementation of this mutant with the wild-type gene. The lvhB2 mutant does not display a very obvious defect in interactions with host cells when the bacteria are grown at 37 degrees C, but it has an approximately 100-fold effect on entry and intracellular replication when grown at 30 degrees C. These data suggest that lvhB2 plays an important role in the efficiency of host cell infection by L. pneumophila grown at lower temperatures.
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Registered trials
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