ArticleGenetics2003
Schizosaccharomyces pombe Ras1 effector, Scd1, interacts with Klp5 and Klp6 kinesins to mediate cytokinesis.
Article in Genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Functional and structural insights into RAS effector proteins.Molecular cell · 2024Review
- Kinesin-8 and Dis1/TOG collaborate to limit spindle elongation from prophase to anaphase A for proper chromosome segregation in fission yeast.Journal of cell science · 2019Article
- Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast.Molecular biology of the cell · 2016Article
- Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.BMC genomics · 2012Article
- Ras signaling in yeast.Genes & cancer · 2011Article
- Two CDC42 paralogues modulate Cryptococcus neoformans thermotolerance and morphogenesis under host physiological conditions.Molecular microbiology · 2010Article
- Int6 and Moe1 interact with Cdc48 to regulate ERAD and proper chromosome segregation.Cell cycle (Georgetown, Tex.) · 2010Article
- Kinesin-8 from fission yeast: a heterodimeric, plus-end-directed motor that can couple microtubule depolymerization to cargo movement.Molecular biology of the cell · 2009Article
- Fission yeast kinesin-8 Klp5 and Klp6 are interdependent for mitotic nuclear retention and required for proper microtubule dynamics.Molecular biology of the cell · 2008Article
- Novel interactions of fission yeast kinesin 8 revealed through in vivo expression of truncation alleles.Cell motility and the cytoskeleton · 2008Article
- The meiotic bouquet promotes homolog interactions and restricts ectopic recombination in Schizosaccharomyces pombe.Genetics · 2006Article
- Compartmentalized signaling of Ras in fission yeast.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Fission yeast Scd1 is an exchange factor for Cdc42 and an effector of Ras1. In a screen for scd1 interacting genes, we isolated klp5 and klp6, which encode presumptive kinesins. Klp5 and Klp6 form a complex to control the same processes, which so far include microtubule dynamics and chromosome segregation. We showed that klp5 or klp6 inactivation in combination with the scd1 deletion (scd1delta) created a synthetic temperature-dependent growth defect. Further genetic analysis demonstrated that Klp5 and Klp6 interacted specifically with the Ras1-Scd1 pathway, but not with the Ras1-Byr2 pathway. In addition, Klp5 and Klp6 can stably associate with Scd1 and Cdc42. A deletion in the Scd1 C terminus, which contains the PB1 domain, prevented Scd1 binding to Klp5/6 and caused a growth defect in Klp5/6 mutant cells that is indistinguishable from that induced by scd1delta. Analysis of the double-mutant phenotype indicated that at the nonpermissive temperature, cells failed to undergo cytokinesis efficiently. These cells contained abnormal contractile rings in which F-actin and Mid1, a key regulator of F-actin ring formation and positioning, are mispositioned and fragmented. These data suggest that Klp5/6 cooperate with the Ras1-Scd1 pathway to influence proper formation of the contractile ring for cytokinesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.