ArticleGenetics2003
Recombinogenic effects of DNA-damaging agents are synergistically increased by transcription in Saccharomyces cerevisiae. New insights into transcription-associated recombination.
Article in Genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Spontaneous deamination of cytosine to uracil is biased to the non-transcribed DNA strand in yeast.DNA repair · 2023Article
- Breaking the paradigm: early insights from mammalian DNA breakomes.The FEBS journal · 2022Review
- Evaluating the Genomic Parameters Governing rAAV-Mediated Homologous Recombination.Molecular therapy : the journal of the American Society of Gene Therapy · 2021Article
- Yra1-bound RNA-DNA hybrids cause orientation-independent transcription-replication collisions and telomere instability.Genes & development · 2018Article
- A new role for Rrm3 in repair of replication-born DNA breakage by sister chromatid recombination.PLoS genetics · 2017Article
- Review
- Transcription and recombination: when RNA meets DNA.Cold Spring Harbor perspectives in biology · 2014Review
- Histone H3K56 acetylation, Rad52, and non-DNA repair factors control double-strand break repair choice with the sister chromatid.PLoS genetics · 2013Article
- Article
- High levels of transcription stimulate transversions at GC base pairs in yeast.Environmental and molecular mutagenesis · 2013Article
- Transcription as a source of genome instability.Nature reviews. Genetics · 2012Review
- Topological constraints impair RNA polymerase II transcription and causes instability of plasmid-borne convergent genes.Nucleic acids research · 2012Article
- Genome-wide function of THO/TREX in active genes prevents R-loop-dependent replication obstacles.The EMBO journal · 2011Article
- Role for topoisomerase 1 in transcription-associated mutagenesis in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Abasic sites in the transcribed strand of yeast DNA are removed by transcription-coupled nucleotide excision repair.Molecular and cellular biology · 2010Article
- The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants.Molecular and cellular biology · 2009Article
- Article
- Transcription-associated recombination is independent of XRCC2 and mechanistically separate from homology-directed DNA double-strand break repair.Nucleic acids research · 2009Article
- Spt2p defines a new transcription-dependent gross chromosomal rearrangement pathway.PLoS genetics · 2008Article
- Nap1 links transcription elongation, chromatin assembly, and messenger RNP complex biogenesis.Molecular and cellular biology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homologous recombination of a particular DNA sequence is strongly stimulated by transcription, a phenomenon observed from bacteria to mammals, which we refer to as transcription-associated recombination (TAR). TAR might be an accidental feature of DNA chemistry with important consequences for genetic stability. However, it is also essential for developmentally regulated processes such as class switching of immunoglobulin genes. Consequently, it is likely that TAR embraces more than one mechanism. In this study we tested the possibility that transcription induces recombination by making DNA more susceptible to recombinogenic DNA damage. Using different plasmid-chromosome and direct-repeat recombination constructs in which transcription is driven from either the P(GAL1)- or the P(tet)-regulated promoters, we have shown that either 4-nitroquinoline-N-oxide (4-NQO) or methyl methanesulfonate (MMS) produces a synergistic increase of recombination when combined with transcription. 4-NQO and MMS stimulated recombination of a transcriptionally active DNA sequence up to 12,800- and 130-fold above the spontaneous levels observed in the absence of transcription, whereas 4-NQO and MMS alone increased recombination 193- and 4.5-fold, respectively. Our results provide evidence that TAR is due, at least in part, to the ability of transcription to enhance the accessibility of DNA to exogenous chemicals and internal metabolites responsible for recombinogenic lesions. We discuss possible parallelisms between the mechanisms of induction of recombination and mutation by transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.