Evidence map›Paper›PMID 14570195›Full record

ArticleAnalytical chemistry2003

Metallic colloid wavelength-ratiometric scattering sensors.

David Roll, Joanna Malicka, Ignacy Gryczynski, Zygmunt Gryczynski, Joseph R Lakowicz

Open access · greenAbstract read
In one paragraph

Article in Analytical chemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 83 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

David RollCenter for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA.
Joanna Malicka
Ignacy Gryczynski
Zygmunt Gryczynski
Joseph R Lakowicz
University of Maryland, Baltimore · US

Funding

WHO'S WHO IN FLUORESCENCEP41RR008119 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI BASKAKOV, ILIA V · 1993 to 2006
$7.4M
Metal-Enhanced Fluorescence SensingR01EB000682 · NIBIB · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2002 to 2006
$1.7M
NCRR NIH HHS P41 RR008119NCRR NIH HHS RR-08119NIBIB NIH HHS EB-00682NIBIB NIH HHS R01 EB000682
6 · The paper itself

Abstract

Gold and silver colloids display strong colors as a result of electron oscillations induced by incident light, which are referred to as the plasmon absorption. This absorption is dependent on colloid-colloid proximity, which has been the basis of absorption assays using colloids. We now describe a new approach to optical sensing using the light scattering properties of colloids. Colloid aggregation was induced by avidin-biotin interactions, which shifted the plasmon absorption to longer wavelengths. We found the spectral shift results in changes in the scattering at different incident wavelengths. By measuring the ratio of scattered intensities at two incident wavelengths, this measurement was made independent of the total colloid concentration. The high scattering efficiency of the colloids resulted in intensities equivalent to fluorescence when normalized by the optical density of the fluorophore and colloid. This approach can be used in a wide variety of assay formats, including those commonly used with fluorescence detection.

Indexed as

Affinity LabelsAvidinBiotinColloidsGold ColloidLightMetalsRhodaminesScattering, RadiationSurface Plasmon ResonanceAffinity LabelsAvidinBiotinColloidsGold ColloidMetalsrhodamine BRhodamines

Identifiers

PMID14570195
PMCPMC2729164
OpenAlexW2072319131

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.