ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2003
Immunocytochemical detection of GLUT2 at the rat intestinal brush-border membrane.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 73 citations in OpenAlex.
- Cryptosporidium parvumcompetes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves.Veterinary research · 2023Article
- Regulation of Glucose Insulinotropic Peptide and Intestinal Glucose Transporters in the Diet-Induced Obese Mouse.Journal of diabetes research · 2022Article
- Resistant starch: Implications of dietary inclusion on gut health and growth in pigs: a review.Journal of animal science and biotechnology · 2021Review
- Nutrient-Induced Cellular Mechanisms of Gut Hormone Secretion.Nutrients · 2021Review
- Computational Modelling of Glucose Uptake by SGLT1 and Apical GLUT2 in the Enterocyte.Frontiers in physiology · 2021Article
- Glucose transporters in the small intestine in health and disease.Pflugers Archiv : European journal of physiology · 2020Review
- The Fast Lane of Hypoxic Adaptation: Glucose Transport Is Modulated via A HIF-Hydroxylase-AMPK-Axis in Jejunum Epithelium.International journal of molecular sciences · 2019Article
- Intestinal Saturated Long-Chain Fatty Acid, Glucose and Fructose Transporters and Their Inhibition by Natural Plant Extracts in Caco-2 Cells.Molecules (Basel, Switzerland) · 2018Review
- Review
- Live imaging of GLUT2 glucose-dependent trafficking and its inhibition in polarized epithelial cysts.Open biology · 2014Article
- Effect of the artificial sweetener, acesulfame potassium, a sweet taste receptor agonist, on glucose uptake in small intestinal cell lines.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2013Article
- Translocation of transfected GLUT2 to the apical membrane in rat intestinal IEC-6 cells.Digestive diseases and sciences · 2012Article
- Acute enterocyte adaptation to luminal glucose: a posttranslational mechanism for rapid apical recruitment of the transporter GLUT2.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2012Article
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- Peptide absorption after massive proximal small bowel resection: mechanisms of ileal adaptation.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2011Article
- Absence of evidence of translocation of GLUT2 to the apical membrane of enterocytes in everted intestinal sleeves.The Journal of surgical research · 2011Article
- Review
- Sodium/glucose cotransporter-1, sweet receptor, and disaccharidase expression in the intestine of the domestic dog and cat: two species of different dietary habit.American journal of physiology. Regulatory, integrative and comparative physiology · 2011Article
- Dietary fructose causes tubulointerstitial injury in the normal rat kidney.American journal of physiology. Renal physiology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have proposed a new model of intestinal sugar absorption in which high sugar concentrations promote rapid insertion of the facilitative transporter GLUT2 into the brush-border membrane so that absorptive capacity is precisely regulated to match dietary intake during the assimilation of a meal. However, location of GLUT2 at the brush border by immunocytochemistry has been problematical. We report that control of rapid GLUT2 trafficking and the use of an antibody to a sequence within the large extracellular loop of GLUT2 permits localization of GLUT2 at the brush border. To reveal brush-border GLUT2 fully, it is necessary to digest the sugar chain at the glycosylation site close to the antigenic site. In this way, we have demonstrated by immunocytochemistry PKC-dependent changes in the regulation of brush-border GLUT2 in rat jejunum that correspond to those seen by Western blotting. The functional and immunocytochemical data are now reconciled.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.