ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2003
C-Met expression and mechanical activation of satellite cells on cultured muscle fibers.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed.
- A Novel Method for Thyroarytenoid Myofiber Culture.The Laryngoscope · 2023Article
- Advanced Methodology for Rapid Isolation of Single Myofibers from Flexor Digitorum Brevis Muscle.Tissue engineering. Part C, Methods · 2023Article
- Electrically Triggered Quercetin Release from Polycaprolactone/Bismuth Ferrite Microfibrous Scaffold for Skeletal Muscle Tissue.Pharmaceutics · 2023Article
- Article
- Key concepts in muscle regeneration: muscle "cellular ecology" integrates a gestalt of cellular cross-talk, motility, and activity to remodel structure and restore function.European journal of applied physiology · 2022Review
- Reduced growth rate of aged muscle stem cells is associated with impaired mechanosensitivity.Aging · 2022Article
- "Time window" effect of Yoda1-evoked Piezo1 channel activity during mouse skeletal muscle differentiation.Acta physiologica (Oxford, England) · 2021Article
- Fusion and beyond: Satellite cell contributions to loading-induced skeletal muscle adaptation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Review
- Myofiber stretch induces tensile and shear deformation of muscle stem cells in their native niche.Biophysical journal · 2021Article
- The Satellite Cell at 60: The Foundation Years.Journal of neuromuscular diseases · 2021Review
- Sarcopenia: Molecular Pathways and Potential Targets for Intervention.International journal of molecular sciences · 2020Review
- Traction and attraction: haptotaxis substrates collagen and fibronectin interact with chemotaxis by HGF to regulate myoblast migration in a microfluidic device.American journal of physiology. Cell physiology · 2020Article
- Hepatocyte growth factor acts as a mitogen for equine satellite cells via protein kinase C δ-directed signaling.Journal of animal science · 2018Article
- Post-injury stretch promotes recovery in a rat model of muscle damage induced by lengthening contractions.The journal of physiological sciences : JPS · 2018Article
- A mechanism for semaphorin-induced apoptosis: DNA damage of endothelial and myogenic cells in primary cultures from skeletal muscle.Oncotarget · 2018Article
- Characterization and utilization of the flexor digitorum brevis for assessing skeletal muscle function.Skeletal muscle · 2018Article
- Article
- Review
- Isolation, Culture, and Immunostaining of Skeletal Muscle Myofibers from Wildtype and Nestin-GFP Mice as a Means to Analyze Satellite Cell.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Adapted physical exercise enhances activation and differentiation potential of satellite cells in the skeletal muscle of old mice.Journal of anatomy · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Single-fiber cultures can be used to model satellite cell activation in vivo. Although technical deficiencies previously prevented study of stretch-induced events, here we describe a method developed to study satellite cell gene expression by in situ hybridization (ISH) using protocol modifications for fiber adhesion and fixation. The hypothesis that mechanical stretching activates satellite cells was tested. Fiber cultures were established from normal flexor digitorum brevis muscles and plated on FlexCell dishes with a layer of Vitrogen. After 2 hr of stretch in the presence of BrdU, satellite cells on fibers attached to Vitrogen were activated above control levels. In the absence of activating treatments or mechanical stretch, ISH studies showed 0-6 c-Met+ satellite cells per fiber. Time course experiments demonstrated stable quiescence in the absence of stretch and significant peaks in activation after 30 min and 2 hr of stretch. Frequency distributions for unstretched fiber cultures showed a significantly greater number of quiescent c-Met+ satellite cells than were activated by stretching, suggesting that typical activation stimuli did not trigger cycling in the entire c-Met+ population of satellite cells. These methods have a strong potential to further dissect the nature of stretch-induced activation and gene expression among characterized populations of individual quiescent and activated satellite cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.