ArticleMolecular biology of the cell2004
Lipid-dependent bidirectional traffic of apolipoprotein B in polarized enterocytes.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Glucagon-like peptide-2 regulates release of chylomicrons from the intestine.Gastroenterology · 2014Trial
- Enterocytes rely on purine biosynthesis/salvage pathway to facilitate dietary fat absorption.Nature communications · 2026Article
- Phosphate Improves Mitochondrial Function and Reduces Pancreatitis in Hypertriglyceridemia.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- NMDA Suppresses Pancreatic ABCA1 Expression through the MEK/ERK/LXR Pathway in Pancreatic Beta Cells.Nutrients · 2024Article
- Article
- Regulation of intestinal lipid metabolism: current concepts and relevance to disease.Nature reviews. Gastroenterology & hepatology · 2020Review
- ApoB48 as an Efficient Regulator of Intestinal Lipid Transport.Frontiers in physiology · 2020Article
- Caco-2 Cells for Measuring Intestinal Cholesterol Transport - Possibilities and Limitations.Biological procedures online · 2020Article
- Trehalose itself plays a critical role on lipid metabolism: Trehalose increases jejunum cytoplasmic lipid droplets which negatively correlated with mesenteric adipocyte size in both HFD-fed trehalase KO and WT mice.Nutrition & metabolism · 2020Article
- Regulation of Chylomicron Secretion: Focus on Post-Assembly Mechanisms.Cellular and molecular gastroenterology and hepatology · 2019Review
- Angiotensin II induces cholesterol accumulation and impairs insulin secretion by regulating ABCA1 in beta cells.Journal of lipid research · 2018Article
- Specific cellular incorporation of a pyrene-labelled cholesterol: lipoprotein-mediated delivery toward ordered intracellular membranes.PloS one · 2015Article
- Autophagosomes contribute to intracellular lipid distribution in enterocytes.Molecular biology of the cell · 2014Article
- Scavenger receptor class B type I is a plasma membrane cholesterol sensor.Circulation research · 2013Article
- Gut triglyceride production.Biochimica et biophysica acta · 2012Article
- The proteome of cytosolic lipid droplets isolated from differentiated Caco-2/TC7 enterocytes reveals cell-specific characteristics.Biology of the cell · 2011Article
- Article
- Adaptation of enterocytic Caco-2 cells to glucose modulates triacylglycerol-rich lipoprotein secretion through triacylglycerol targeting into the endoplasmic reticulum lumen.The Biochemical journal · 2006Article
- Bovine prion is endocytosed by human enterocytes via the 37 kDa/67 kDa laminin receptor.The American journal of pathology · 2005Article
- Characterization of a spontaneously polarizing HT-29 cell line, HT-29/cl.f8.In vitro cellular & developmental biology. AnimalArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterocytes are highly polarized cells that transfer nutrients across the intestinal epithelium from the apical to the basolateral pole. Apolipoprotein B (apoB) is a secretory protein that plays a key role in the transepithelial transport of dietary fatty acids as triacylglycerol. The evaluation of the control of apoB traffic by lipids is therefore of particular interest. To get a dynamic insight into this process, we used the enterocytic Caco-2 cells cultured on microporous filters, a system in which the apical and basal compartments can be delimited. Combining biochemical and morphological approaches, our results showed that, besides their role in protection from degradation, lipids control the intracellular traffic of apoB in enterocytes. A supply of fatty acids and cholesterol is sufficient for the export of apoB from the endoplasmic reticulum and its post-Golgi traffic up to the apical brush-border domain, where it remains until an apical supply of complex lipid micelles signals its chase down to the basolateral secretory domain. This downward traffic of apoB involves a microtubule-dependent process. Our results demonstrate an enterocyte-specific bidirectional process for the lipid-dependent traffic of a secretory protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.