ArticleMolecular biology of the cell2003
Dissection of the Ascaris sperm motility machinery identifies key proteins involved in major sperm protein-based amoeboid locomotion.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- RNAi-dependent expression of sperm genes in ADL chemosensory neurons is required for olfactory responses inFrontiers in molecular biosciences · 2024Article
- NHR-23 and SPE-44 regulate distinct sets of genes during Caenorhabditis elegans spermatogenesis.G3 (Bethesda, Md.) · 2022Article
- Spatial Transcriptomics of Nematodes Identifies Sperm Cells as a Source of Genomic Novelty and Rapid Evolution.Molecular biology and evolution · 2021Article
- MFP1/MSD-1 and MFP2/NSPH-2 co-localize with MSP duringmicroPublication biology · 2021Article
- Liver fluke infection and cholangiocarcinoma: a review.Parasitology research · 2017Review
- Article
- Extending the molecular clutch beyond actin-based cell motility.New journal of physics · 2014Article
- The regulation of spermatogenesis and sperm function in nematodes.Seminars in cell & developmental biology · 2014Review
- Article
- Spermatogenesis.Advances in experimental medicine and biology · 2013Review
- Transformation: how do nematode sperm become activated and crawl?Protein & cell · 2012Review
- An analysis of the transcriptome of Teladorsagia circumcincta: its biological and biotechnological implications.BMC genomics · 2012Article
- Comparative transcriptome sequencing of germline and somatic tissues of the Ascaris suum gonad.BMC genomics · 2011Article
- Article
- Evidence for phosphorylation in the MSP cytoskeletal filaments of amoeboid spermatozoa.International journal of biochemistry and molecular biology · 2011Article
- New insights into the mechanism of fertilization in nematodes.International review of cell and molecular biology · 2011Review
- Biochemical mechanisms for regulating protrusion by nematode major sperm protein.Biophysical journal · 2009Article
- Dephosphorylation of major sperm protein (MSP) fiber protein 3 by protein phosphatase 2A during cell body retraction in the MSP-based amoeboid motility of Ascaris sperm.Molecular biology of the cell · 2009Article
- Depolymerization-driven flow in nematode spermatozoa relates crawling speed to size and shape.Biophysical journal · 2008Article
- A Ser/Thr kinase required for membrane-associated assembly of the major sperm protein motility apparatus in the amoeboid sperm of Ascaris.Molecular biology of the cell · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Although Ascaris sperm motility closely resembles that seen in many other types of crawling cells, the lamellipodial dynamics that drive movement result from modulation of a cytoskeleton based on the major sperm protein (MSP) rather than actin. The dynamics of the Ascaris sperm cytoskeleton can be studied in a cell-free in vitro system based on the movement of plasma membrane vesicles by fibers constructed from bundles of MSP filaments. In addition to ATP, MSP, and a plasma membrane protein, reconstitution of MSP motility in this cell-free extract requires cytosolic proteins that orchestrate the site-specific assembly and bundling of MSP filaments that generates locomotion. Here, we identify a fraction of cytosol that is comprised of a small number of proteins but contains all of the soluble components required to assemble fibers. We have purified two of these proteins, designated MSP fiber proteins (MFPs) 1 and 2 and demonstrated by immunolabeling that both are located in the MSP cytoskeleton in cells and in fibers. These proteins had reciprocal effects on fiber assembly in vitro: MFP1 decreased the rate of fiber growth, whereas MFP2 increased the growth rate.
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