ArticleMolecular biology of the cell2004
The Caenorhabditis elegans microtubule-severing complex MEI-1/MEI-2 katanin interacts differently with two superficially redundant beta-tubulin isotypes.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 67 citations in OpenAlex.
- Differential contributions of β-tubulin isotypes to acentrosomal oocyte meiosis in C. elegans.The Journal of cell biology · 2026Article
- Evaluating beta-tubulin variants as predictors of benzimidazole resistance across Caenorhabditis nematodes.PLoS pathogens · 2026Article
- KATANIN promotes cell elongation and division to generate proper cell numbers in maize organs.Nature communications · 2026Article
- Differential contributions of β-tubulin isotypes to acentrosomal oocyte meiosis inbioRxiv : the preprint server for biology · 2025Article
- Microtubule-binding domains in Katanin p80 subunit are essential for severing activity in C. elegans.The Journal of cell biology · 2024Article
- Interactions of Caenorhabditis elegans β-tubulins with the microtubule inhibitor and anthelmintic drug albendazole.Genetics · 2022Article
- STRIPAK regulation of katanin microtubule severing in the Caenorhabditis elegans embryo.Genetics · 2022Article
- The Expression and Function of Tubulin Isotypes inFrontiers in cell and developmental biology · 2022Review
- Tubulin isotypes optimize distinct spindle positioning mechanisms during yeast mitosis.The Journal of cell biology · 2021Article
- Lentiviral transduction facilitates RNA interference in the nematode parasite Nippostrongylus brasiliensis.PLoS pathogens · 2021Article
- Phosphorylation of the microtubule-severing AAA+ enzyme Katanin regulates C. elegans embryo development.The Journal of cell biology · 2020Article
- An allosteric network in spastin couples multiple activities required for microtubule severing.Nature structural & molecular biology · 2019Article
- Extreme allelic heterogeneity at a Caenorhabditis elegans beta-tubulin locus explains natural resistance to benzimidazoles.PLoS pathogens · 2018Article
- Microtubule Dynamics Scale with Cell Size to Set Spindle Length and Assembly Timing.Developmental cell · 2018Article
- Differential regulation of polarized synaptic vesicle trafficking and synapse stability in neural circuit rewiring in Caenorhabditis elegans.PLoS genetics · 2017Article
- Posttranslational Modifications of Tubulin and Cilia.Cold Spring Harbor perspectives in biology · 2017Review
- Regulation of the MEI-1/MEI-2 Microtubule-Severing Katanin Complex in Early Caenorhabditis elegans Development.G3 (Bethesda, Md.) · 2016Article
- Article
- Caenorhabditis elegans oocyte meiotic spindle pole assembly requires microtubule severing and the calponin homology domain protein ASPM-1.Molecular biology of the cell · 2014Article
- Microtubule severing by the katanin complex is activated by PPFR-1-dependent MEI-1 dephosphorylation.The Journal of cell biology · 2013Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microtubule-severing protein complex katanin is required for a variety of important microtubule-base morphological changes in both animals and plants. Caenorhabditis elegans katanin is encoded by the mei-1 and mei-2 genes and is required for oocyte meiotic spindle formation and must be inactivated before the first mitotic cleavage. We identified a mutation, sb26, in the tbb-2 beta-tubulin gene that partially inhibits MEI-1/MEI-2 activity: sb26 rescues lethality caused by ectopic MEI-1/MEI-2 expression during mitosis, and sb26 increases meiotic defects in a genetic background where MEI-1/MEI-2 activity is lower than normal. sb26 does not interfere with MEI-1/MEI-2 microtubule localization, suggesting that this mutation likely interferes with severing. Tubulin deletion alleles and RNA-mediated interference revealed that TBB-2 and the other germline enriched beta-tubulin isotype, TBB-1, are redundant for embryonic viability. However, limiting MEI-1/MEI-2 activity in these experiments revealed that MEI-1/MEI-2 preferentially interacts with TBB-2-containing microtubules. Our results demonstrate that these two superficially redundant beta-tubulin isotypes have functionally distinct roles in vivo.
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