ArticleMolecular biology of the cell2004
Mhr1p-dependent concatemeric mitochondrial DNA formation for generating yeast mitochondrial homoplasmic cells.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 71 citations in OpenAlex.
- Sexual life cycle establishes the unicellular red algae Cyanidiophyceae as a genetically tractable model for eukaryotic evolution.The Plant cell · 2026Article
- Caloric restriction enhances inheritance of wild-type mitochondrial DNA in Saccharomyces cerevisiae.Scientific reports · 2025Article
- Inheritance bias of deletion-harbouring mtDNA in yeast: The role of copy number and intracellular selection.PLoS genetics · 2025Article
- Two mitochondrial HMG-box proteins, Cim1 and Abf2, antagonistically regulate mtDNA copy number in Saccharomyces cerevisiae.Nucleic acids research · 2023Article
- petiteFinder: an automated computer vision tool to compute Petite colony frequencies in baker's yeast.BMC bioinformatics · 2023Article
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- A novel fragmented mitochondrial genome in the protist pathogenGenome research · 2021Article
- Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi.Nature communications · 2020Article
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- Diversity and Postzygotic Evolution of the Mitochondrial Genome in Hybrids ofFrontiers in microbiology · 2020Article
- Prevention of mitochondrial genomic instability in yeast by the mitochondrial recombinase Mhr1.Scientific reports · 2019Article
- Saccharomyces cerevisiae Mhr1 can bind Xho I-induced mitochondrial DNA double-strand breaks in vivo.Mitochondrion · 2018Article
- Regulation of Small Mitochondrial DNA Replicative Advantage by Ribonucleotide Reductase inG3 (Bethesda, Md.) · 2017Article
- Evidence for double-strand break mediated mitochondrial DNA replication in Saccharomyces cerevisiae.Nucleic acids research · 2017Article
- Organization and dynamics of yeast mitochondrial nucleoids.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2017Review
- Reactive oxygen species stimulate mitochondrial allele segregation toward homoplasmy in human cells.Molecular biology of the cell · 2016Article
- A rolling circle replication mechanism produces multimeric lariats of mitochondrial DNA in Caenorhabditis elegans.PLoS genetics · 2015Article
- Double-stranded DNA-dependent ATPase Irc3p is directly involved in mitochondrial genome maintenance.Nucleic acids research · 2014Article
- Mechanism of homologous recombination and implications for aging-related deletions in mitochondrial DNA.Microbiology and molecular biology reviews : MMBR · 2013Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondria carry many copies of mitochondrial DNA (mtDNA), but mt-alleles quickly segregate during mitotic growth through unknown mechanisms. Consequently, all mtDNA copies are often genetically homogeneous within each individual ("homoplasmic"). Our previous study suggested that tandem multimers ("concatemers") formed mainly by the Mhr1p (a yeast nuclear gene-encoded mtDNA-recombination protein)-dependent pathway are required for mtDNA partitioning into buds with concomitant monomerization. The transmission of a few randomly selected clones (as concatemers) of mtDNA into buds is a possible mechanism to establish homoplasmy. The current study provides evidence for this hypothesis as follows: the overexpression of MHR1 accelerates mt-allele-segregation in growing heteroplasmic zygotes, and mhr1-1 (recombination-deficient) causes its delay. The mt-allele-segregation rate correlates with the abundance of concatemers, which depends on Mhr1p. In G1-arrested cells, concatemeric mtDNA was labeled by [14C]thymidine at a much higher density than monomers, indicating concatemers as the immediate products of mtDNA replication, most likely in a rolling circle mode. After releasing the G1 arrest in the absence of [14C]thymidine, the monomers as the major species in growing buds of dividing cells bear a similar density of 14C as the concatemers in the mother cells, indicating that the concatemers in mother cells are the precursors of the monomers in buds.
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