ArticleJournal of bacteriology2003
A mutant of Paracoccus denitrificans with disrupted genes coding for cytochrome c550 and pseudoazurin establishes these two proteins as the in vivo electron donors to cytochrome cd1 nitrite reductase.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 61 citations in OpenAlex.
- Deep learning-drivenSynthetic and systems biotechnology · 2027Article
- Biochemical Characterization of the Copper Nitrite Reductase fromBiomolecules · 2023Article
- Genetic diversity into a novel free-living species ofFrontiers in microbiology · 2023Article
- Metabolic Homeostasis in Life as We Know It: Its Origin and Thermodynamic Basis.Frontiers in physiology · 2021Article
- The crystal structure of the heme dActa crystallographica. Section D, Structural biology · 2020Article
- Genomic and Metabolic Insights into Denitrification, Sulfur Oxidation, and Multidrug Efflux Pump Mechanisms in the BacteriumMicroorganisms · 2020Article
- Functional interactions between nitrite reductase and nitric oxide reductase from Paracoccus denitrificans.Scientific reports · 2019Article
- Bacterial periplasmic nitrate and trimethylamine-N-oxide respiration coupled to menaquinol-cytochrome c reductase (Qcr): Implications for electrogenic reduction of alternative electron acceptors.Scientific reports · 2018Article
- Recent advances in the biosynthesis of modified tetrapyrroles: the discovery of an alternative pathway for the formation of heme and heme d 1.Cellular and molecular life sciences : CMLS · 2014Article
- Transferable denitrification capability of Thermus thermophilus.Applied and environmental microbiology · 2014Review
- Bacterial adaptation of respiration from oxic to microoxic and anoxic conditions: redox control.Antioxidants & redox signaling · 2012Review
- The electron transfer complex between nitrous oxide reductase and its electron donors.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2011Article
- Identification, functional studies, and genomic comparisons of new members of the NnrR regulon in Rhodobacter sphaeroides.Journal of bacteriology · 2010Article
- Agrobacterium tumefaciens C58 uses ActR and FnrN to control nirK and nor expression.Journal of bacteriology · 2008Article
- A new assay for nitric oxide reductase reveals two conserved glutamate residues form the entrance to a proton-conducting channel in the bacterial enzyme.The Biochemical journal · 2007Article
- Maturation of the unusual single-cysteine (XXXCH) mitochondrial c-type cytochromes found in trypanosomatids must occur through a novel biogenesis pathway.The Biochemical journal · 2004Article
- Wide-range transcriptional modulating effect of ntrR under microaerobiosis in Sinorhizobium meliloti.Molecular genetics and genomics : MGG · 2004Article
- Denitrification genes regulate Brucella virulence in mice.Journal of bacteriology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Paracoccus denitrificans, electrons pass from the membrane-bound cytochrome bc(1) complex to the periplasmic nitrite reductase, cytochrome cd(1). The periplasmic protein cytochrome c(550) has often been implicated in this electron transfer, but its absence, as a consequence of mutation, has previously been shown to result in almost no attenuation in the ability of the nitrite reductase to function in intact cells. Here, the hypothesis that cytochrome c(550) and pseudoazurin are alternative electron carriers from the cytochrome bc(1) complex to the nitrite reductase was tested by construction of mutants of P. denitrificans that are deficient in either pseudoazurin or both pseudoazurin and cytochrome c(550). The latter organism, but not the former (which is almost indistinguishable in this respect from the wild type), grows poorly under anaerobic conditions with nitrate as an added electron acceptor and accumulates nitrite in the medium. Growth under aerobic conditions with either succinate or methanol as the carbon source is not significantly affected in mutants lacking either pseudoazurin or cytochrome c(550) or both these proteins. We concluded that pseudoazurin and cytochrome c(550) are the alternative electron mediator proteins between the cytochrome bc(1) complex and the cytochrome cd(1)-type nitrite reductase. We also concluded that expression of pseudoazurin is mainly controlled by the transcriptional activator FnrP.
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