ArticleWorld journal of gastroenterology2003
Rapid and high throughput detection of HBV YMDD mutants with fluorescence polarization.
Article in World journal of gastroenterology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Antiviral therapies: focus on hepatitis B reverse transcriptase.The international journal of biochemistry & cell biology · 2012Review
- Article
- Quantitative detection of the M204V hepatitis B virus minor variants by amplification refractory mutation system real-time PCR combined with molecular beacon technology.Journal of clinical microbiology · 2009Article
- Identification of a key functional region in harpins from Xanthomonas that suppresses protein aggregation and mediates harpin expression in E. coli.Molecular biology reports · 2007Article
- Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA.World journal of gastroenterology · 2006Article
- Helicobacter pylori infection in the pharynx of patients with chronic pharyngitis detected with TDI-FP and modified Giemsa stain.World journal of gastroenterology · 2006Article
- Rapid and accurate genotyping of YMDD motif variants in the hepatitis B virus genome by an improved reverse dot blot method.Journal of clinical microbiology · 2005Article
- Detection of hepatitis B virus DNA by real-time PCR using TaqMan-MGB probe technology.World journal of gastroenterology · 2005Article
- Advances in Molecular Diagnosis of HBV Infection and Drug Resistance.International journal of medical sciences · 2005Article
- Effect of SEN virus coinfection on outcome of lamivudine therapy in patients with hepatitis B.World journal of gastroenterology · 2004Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo develop a simple and rapid detection of HBV gene variants and prediction of lamivudine-resistance in patients.
methodsInitially, plasmids harboring the wild-type or mutant HBV DNA fragments were used in a model system. The technique was then applied to clinical samples for an analysis of YMDD mutations. The sera were extracted from chronic hepatitis patients who had received lamivudine treatment for more than one year. P region gene of HBV was amplified by polymerase chain reaction. The excess primers and dNTPs in PCR products were removed by cleaning-up reagents. Template-directed dye-terminator incorporation reaction was performed and R110 or TAMRA labeled acyclo-terminator was added on the 3' end of TDI-primer specifically. Fluorescence polarization value was measured with Victor 2 multilabel counter and the genotypes of HBV were analyzed.
resultsThe YMDD genotypes in recombined positive plasmid and 56 serum samples of HBV infected patients were analyzed by using our TDI-FP method and the specificity and sensitivity were confirmed by DNA sequencing. Five of 56 serum samples showed YVDD phenotype (9%), including 1 YMDD and YVDD mixed infection. Four of 56 showed YIDD phenotype (7.1%).
conclusionThis is a simple, rapid, low cost and high throughput assay to detect HBV polymerase gene variants and suitable for large-scale screening and prediction of the lamivudine-resistance in clinical samples.
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