ArticleWorld journal of gastroenterology2003
Inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma cells in vitro.
Article in World journal of gastroenterology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 38 citations in OpenAlex.
- Hepatic artery injection of ¹³¹I-labelled metuximab combined with chemoembolization for intermediate hepatocellular carcinoma: a prospective nonrandomized study.European journal of nuclear medicine and molecular imaging · 2012Trial
- The clinicopathological significance and prognostic value of EMMPRIN overexpression in cancers: evidence from 39 cohort studies.Oncotarget · 2017Article
- Silencing of advanced glycosylation and glycosylation and product-specific receptor (RAGE) inhibits the metastasis and growth of non-small cell lung cancer.American journal of translational research · 2017Article
- A chimeric antibody targeting CD147 inhibits hepatocellular carcinoma cell motility via FAK-PI3K-Akt-Girdin signaling pathway.Clinical & experimental metastasis · 2015Article
- ADAM17 is associated with EMMPRIN and predicts poor prognosis in patients with uterine cervical carcinoma.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- High expression of CD147 and MMP-9 is correlated with poor prognosis of triple-negative breast cancer (TNBC) patients.Medical oncology (Northwood, London, England) · 2013Article
- CD147 overexpression is a prognostic factor and a potential therapeutic target in bladder cancer.Medical oncology (Northwood, London, England) · 2011Article
- Transcription factor Sp1 regulates expression of cancer-associated molecule CD147 in human lung cancer.Cancer science · 2010Article
- Association of TMPRSS2 and KLK11 gene expression levels with clinical progression of human prostate cancer.Medical oncology (Northwood, London, England) · 2010Article
- Tumor metabolism of lactate: the influence and therapeutic potential for MCT and CD147 regulation.Future oncology (London, England) · 2010Review
- CD147 expression indicates unfavourable prognosis in prostate cancer.Pathology oncology research : POR · 2009Article
- Downregulation of CD147 expression alters cytoskeleton architecture and inhibits gelatinase production and SAPK pathway in human hepatocellular carcinoma cells.Journal of experimental & clinical cancer research : CR · 2008Article
- Function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome coronavirus.The Journal of infectious diseases · 2005Article
- Establishment and characterization of human hepatocellular carcinoma cell line FHCC-98.World journal of gastroenterology · 2004Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study the inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma(HCC) cells in vitro.
methodsAntisense RNA of HAb18G/CD147 vector PCI-asHAb18G was constructed by reversely inserting HAb18G/CD147 cDNA to eukaryotic expression vector PCI-neo. The HCC cell line HHCC was transfected by PCI-asHAb18G via cation liposome. Expression of HAb18G/CD147 of transfected cells selected by G418 (geneticin) was observed by immuno-histochemical SP staining and FACS (fluorescence activated cell sorting). Gelatin zymography was used to determine the effect of PCI-asHAb18G on reducing secretions of MMP-2 and MMP-9 of the transfected cells. Boyden chamber was employed to test the invasion of HCC cells in vitro.
resultsThe construction of antisense RNA vector PCI-asHAb18G was verified correct by partial nucleotide sequencing and restricted endonuclease digestion. The expression of HAb18G/CD147 in transfected HHCC was inhibited by PCI-asHAb18G. Secretions of MMP-2 and MMP-9 of transfected HHCC were reduced and the invasion of transfected HHCC was inhibited compared to HHCC, respectively.
conclusionInvasion of HCC cells can be inhibited by antisense RNA of HAb18G/CD147. HAb18G/CD147 may be used as a potential target of drugs for anti-invasion and metastasis of HCC.
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