ArticleRNA (New York, N.Y.)2003
RNA-protein interactions promote asymmetric sorting of the ASH1 mRNA ribonucleoprotein complex.
Article in RNA (New York, N.Y.), 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Cellular and molecular mechanisms of asymmetric stem cell division in tissue homeostasis.Genes to cells : devoted to molecular & cellular mechanisms · 2024Review
- Phosphorylation controls the oligomeric state of She2 and mRNA localization in yeast.RNA (New York, N.Y.) · 2023Article
- Walking the line: mechanisms underlying directional mRNA transport and localisation in neurons and beyond.Cellular and molecular life sciences : CMLS · 2021Review
- Asymmetric inheritance of epigenetic states in asymmetrically dividing stem cells.Current opinion in cell biology · 2020Review
- Asymmetric Inheritance of Cell Fate Determinants: Focus on RNA.Non-coding RNA · 2019Review
- Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex.Nature structural & molecular biology · 2017Article
- Structure of a myosin•adaptor complex and pairing by cargo.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Using the Yeast Three-Hybrid System to Identify Proteins that Interact with a Phloem-Mobile mRNA.Frontiers in plant science · 2012Article
- A cytoplasmic complex mediates specific mRNA recognition and localization in yeast.PLoS biology · 2011Article
- Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud.Genes & development · 2010Article
- Multiple Myo4 motors enhance ASH1 mRNA transport in Saccharomyces cerevisiae.The Journal of cell biology · 2010Article
- Formation of She2p tetramers is required for mRNA binding, mRNP assembly, and localization.RNA (New York, N.Y.) · 2009Article
- She3p possesses a novel activity required for ASH1 mRNA localization in Saccharomyces cerevisiae.Eukaryotic cell · 2009Article
- Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p.Molecular biology of the cell · 2009Article
- Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA.EMBO reports · 2008Article
- Combining guilt-by-association and guilt-by-profiling to predict Saccharomyces cerevisiae gene function.Genome biology · 2008Article
- mRNA trafficking in fungi.Molecular genetics and genomics : MGG · 2007Review
- Discovery of a mRNA mitochondrial localization element in Saccharomyces cerevisiae by nonhomologous random recombination and in vivo selection.Nucleic acids research · 2007Article
- Identification of a conserved RNA motif essential for She2p recognition and mRNA localization to the yeast bud.Molecular and cellular biology · 2005Article
- Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae.Molecular biology of the cell · 2004Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, ASH1 mRNA is localized to the tip of daughter cells during anaphase of the cell cycle. ASH1 mRNA localization is dependent on four cis-acting localization elements as well as Myo4p, She2p, and She3p. Myo4p, She2p, and She3p are hypothesized to form a heterotrimeric protein complex that directly transports ASH1 mRNA to daughter cells. She2p is an RNA-binding protein that directly interacts with ASH1 cis-acting localization elements and associates with She3p. Here we report the identification of seven She2p mutants-N36S, R43A, R44A, R52A, R52K, R63A, and R63K-that result in the delocalization of ASH1 mRNA. These mutants are defective for RNA-binding activity but retain the ability to interact with She3p, indicating that a functional She2p RNA-binding domain is not a prerequisite for association with She3p. Furthermore, the nuclear/cytoplasmic distribution for the N36S and R63K She2p mutants is not altered, indicating that nuclear/cytoplasmic trafficking of She2p is independent of RNA-binding activity. Using the N36S and R63K She2p mutants, we observed that in the absence of She2p RNA-binding activity, neither Myo4p nor She3p is asymmetrically sorted to daughter cells. However, in the absence of She2p, Myo4p and She3p can be asymmetrically segregated to daughter cells by artificially tethering mRNA to She3p, implying that the transport and/or anchoring of the Myo4p/She3p complex is dependent on the presence of associated mRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.