ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2003
An unbiased cDNA library prepared from isolated Aplysia sensory neuron processes is enriched for cytoskeletal and translational mRNAs.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
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Who cites it
79 citing papers in PubMed, 176 citations in OpenAlex.
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- Synaptically-targeted long non-coding RNA SLAMR promotes structural plasticity by increasing translation and CaMKII activity.Nature communications · 2024Article
- Crosstalk between ubiquitination and translation in neurodevelopmental disorders.Frontiers in molecular neuroscience · 2024Review
- Is there a localized role for translational quality control?RNA (New York, N.Y.) · 2023Article
- Excess ribosomal protein production unbalances translation in a model of Fragile X Syndrome.Nature communications · 2022Article
- Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins.Nature communications · 2021Article
- Molecular motor protein KIF5C mediates structural plasticity and long-term memory by constraining local translation.Cell reports · 2021Article
- Axonal mRNA translation in neurological disorders.RNA biology · 2021Review
- Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning.Cell reports · 2020Article
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- Pleiotropic Mitochondria: The Influence of Mitochondria on Neuronal Development and Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Review
- High-Throughput Isolation of Cell Protrusions with Single-Cell Precision for Profiling Subcellular Gene Expression.Angewandte Chemie (International ed. in English) · 2019Article
- Local translation in neurons: visualization and function.Nature structural & molecular biology · 2019Review
- Single-cell nanobiopsy reveals compartmentalization of mRNAs within neuronal cells.The Journal of biological chemistry · 2018Article
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- Demonstration of ion channel synthesis by isolated squid giant axon provides functional evidence for localized axonal membrane protein translation.Scientific reports · 2018Article
- CeFra-seq reveals broad asymmetric mRNA and noncoding RNA distribution profiles inRNA (New York, N.Y.) · 2018Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Local protein synthesis is required for long-lasting synapse-specific plasticity in cultured Aplysia sensorimotor synapses. To identify synaptically localized mRNAs, we prepared a cDNA library from isolated sensory neurites. By sequence analysis, we estimate that the library contains 263 distinct mRNAs, with 98 of these mRNAs constituting 70% of all clones. The localized transcripts are enriched for mRNAs encoding cytoskeletal elements and components of the translational machinery. In situ hybridization confirms that the mRNAs for at least eight of these transcripts are present in distal neurites. Immunocytochemistry reveals that serotonin regulates the translation of one of the localized mRNAs, that encoding alpha1-tubulin. Our identification of mRNAs encoding cytoskeletal elements suggests that local protein synthesis is required for the growth of new synaptic connections associated with persistent synaptic strengthening. Our finding of mRNAs encoding components of the translational machinery suggests that local protein synthesis serves to increase the translational capacity of synapses.
Indexed as
Identifiers
14561869PMC6740582W2169284406What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.