ArticleThe Journal of physiology2003
Conditional protein phosphorylation regulates BK channel activity in rat cerebellar Purkinje neurons.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
- CaInternational journal of molecular sciences · 2023Review
- Protein kinase Cγ in cerebellar Purkinje cells regulates CaProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Heterogeneity of intrinsic plasticity in cerebellar Purkinje cells linked with cortical molecular zones.iScience · 2022Article
- Cereblon in health and disease.Pflugers Archiv : European journal of physiology · 2016Review
- An EP2 Agonist Facilitates NMDA-Induced Outward Currents and Inhibits Dendritic Beading through Activation of BK Channels in Mouse Cortical Neurons.Mediators of inflammation · 2016Article
- Increased excitability of medium-sized dorsal root ganglion neurons by prolonged interleukin-1β exposure is K(+) channel dependent and reversible.The Journal of physiology · 2015Article
- Big Potassium (BK) ion channels in biology, disease and possible targets for cancer immunotherapy.International immunopharmacology · 2014Review
- Integration of modeling with experimental and clinical findings synthesizes and refines the central role of inositol 1,4,5-trisphosphate receptor 1 in spinocerebellar ataxia.Frontiers in neuroscience · 2014Review
- Phosphorylation of BK channels modulates the sensitivity to hydrogen sulfide (H2S).Frontiers in physiology · 2014Article
- Stochastic calcium mechanisms cause dendritic calcium spike variability.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Iberiotoxin-sensitive and -insensitive BK currents in Purkinje neuron somata.Journal of neurophysiology · 2013Article
- Hair cell BK channels interact with RACK1, and PKC increases its expression on the cell surface by indirect phosphorylation.American journal of physiology. Cell physiology · 2012Article
- Impairment of neurovascular coupling in type 1 diabetes mellitus in rats is linked to PKC modulation of BK(Ca) and Kir channels.American journal of physiology. Heart and circulatory physiology · 2012Article
- Participation of K(ATP) Channels in the Antinociceptive Effect of Pregabalin in Rat Formalin Test.The Korean journal of pain · 2011Article
- Bidirectional control of BK channel open probability by CAMKII and PKC in medial vestibular nucleus neurons.Journal of neurophysiology · 2011Article
- Dual role of protein kinase C on BK channel regulation.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- BKCa currents are enriched in a subpopulation of adult rat cutaneous nociceptive dorsal root ganglion neurons.The European journal of neuroscience · 2010Article
- Large conductance calcium-activated potassium channels affect both spontaneous firing and intracellular calcium concentration in cerebellar Purkinje neurons.Neuroscience · 2009Article
- Profiling the phospho-status of the BKCa channel alpha subunit in rat brain reveals unexpected patterns and complexity.Molecular & cellular proteomics : MCP · 2008Article
- The BK-mediated fAHP is modulated by learning a hippocampus-dependent task.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large conductance calcium- and voltage-activated potassium (BK) channels are widely expressed in the mammalian central nervous system. Although the activity of BK channels in endocrine and vascular cells is regulated by protein kinases and phosphatases associated with the channel complex, direct evidence for such modulation in neurons is largely lacking. Single-channel analysis from inside-out patches isolated from the soma of dissociated rat cerebellar Purkinje neurons demonstrated that the activity of BK channels is regulated by multiple endogenous protein kinases and protein phosphatases in the membrane patch. The majority of BK channels were non-inactivating and displayed a 'low' activity phenotype determined at +40 mV and 1 muM intracellular free calcium. These channels were activated by cAMP-dependent protein kinase (PKA) associated with the patch and the extent of PKA activation was limited by an opposing endogenous type 2A-like protein phosphatase (PP2A). Importantly, PKA activation was dependent upon the prior phosphorylation status of the BK channel complex dynamically controlled by protein kinase C (PKC) and protein phosphatase 1 (PP1). In contrast, Purkinje cells also displayed a low proportion of non-inactivating BK channels with a 'high' activity under the same recording conditions and these channels were inhibited by endogenous PKA. Our data suggest that: (1) multiple endogenous protein kinases and phosphatases functionally couple to the BK channel complex to allow conditional modulation of BK channel activity in neurons, and (2) native, phenotypically distinct, neuronal BK channels are differentially sensitive to PKA-dependent phosphorylation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.