ArticleThe Biochemical journal2004
Localization of dynein light chains 1 and 2 and their pro-apoptotic ligands.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 74 citations in OpenAlex.
- Bcl-2 modifying factor (Bmf): "a mysterious stranger" in the Bcl-2 family proteins.Cell death and differentiation · 2026Review
- Non-canonical phosphorylation of Bmf by p38 MAPK promotes its apoptotic activity in anoikis.Cell death and differentiation · 2022Article
- DYNLT1 gene expression is downregulated in whole blood of patients at different Huntington's disease stages.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2021Article
- Dynein light chain binding determines complex formation and posttranslational stability of the Bcl-2 family members Bmf and Bim.Cell death and differentiation · 2020Article
- Heterogeneous Nuclear Ribonucleoprotein F Mediates Insulin Inhibition of Bcl2-Modifying Factor Expression and Tubulopathy in Diabetic Kidney.Scientific reports · 2019Article
- Structural atlas of dynein motors at atomic resolution.Biophysical reviews · 2018Review
- The established and the predicted roles of dynein light chain in the regulation of mitochondrial apoptosis.Cell cycle (Georgetown, Tex.) · 2018Article
- Dynein light chain 1 induces assembly of large Bim complexes on mitochondria that stabilize Mcl-1 and regulate apoptosis.Genes & development · 2017Article
- MECHANISMS IN ENDOCRINOLOGY: Alternative splicing: the new frontier in diabetes research.European journal of endocrinology · 2016Review
- Dynein function and protein clearance changes in tumor cells induced by a Kunitz-type molecule, Amblyomin-X.PloS one · 2014Article
- BimEL is phosphorylated at mitosis by Aurora A and targeted for degradation by βTrCP1.Cell death and differentiation · 2013Article
- A myosin-Va tail fragment sequesters dynein light chains leading to apoptosis in melanoma cells.Cell death & disease · 2013Article
- N-terminal cleavage of the mitochondrial fusion GTPase OPA1 occurs via a caspase-independent mechanism in cerebellar granule neurons exposed to oxidative or nitrosative stress.Brain research · 2013Article
- The major isoforms of Bim contribute to distinct biological activities that govern the processes of autophagy and apoptosis in interleukin-7 dependent lymphocytes.Biochimica et biophysica acta · 2012Article
- The Zinc-finger protein ASCIZ regulates B cell development via DYNLL1 and Bim.The Journal of experimental medicine · 2012Article
- An Integrated Bioinformatics and Computational Biology Approach Identifies New BH3-Only Protein Candidates.The open biology journal · 2012Article
- BCL-2 modifying factor (BMF) is a central regulator of anoikis in human intestinal epithelial cells.The Journal of biological chemistry · 2011Article
- Article
- Affinity, avidity, and kinetics of target sequence binding to LC8 dynein light chain isoforms.The Journal of biological chemistry · 2010Article
- Intrinsically disordered proteins in bcl-2 regulated apoptosis.International journal of molecular sciences · 2010Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
The dynein and myosin V motor complexes are multi-protein structures that function to transport molecules and organelles within the cell. DLC (dynein light-chain) proteins, found as components of both dynein and myosin V motor complexes, connect the complexes to their cargoes. One of the roles of these motor complexes is to selectively sequester the pro-apoptotic 'BH3-only' (Bcl-2 homology 3-only) proteins, Bim (Bcl-2-interacting mediator of cell death) and Bmf (Bcl-2-modifying factor), and so regulate their cell death-inducing function. In vivo DLC2 is found exclusively as a component of the myosin V motor complex and Bmf binds DLC2 selectively. On the other hand, Bim interacts with DLC1 (LC8), an integral component of the dynein motor complex. The two DLCs share 93% sequence identity yet show unambiguous in vivo specificity for their respective BH3-only ligands. To investigate this specificity the three-dimensional solution structure of DLC2 was elucidated using NMR spectroscopy. In vitro structural and mutagenesis studies show that Bmf and Bim have identical binding characteristics to recombinant DLC2 or DLC1. Thus the selectivity shown by Bmf and Bim for binding DLC1 or DLC2, respectively, does not reside in their DLC-binding domains. Remarkably, mutational analysis of DLC1 and DLC2 indicates that a single surface residue (residue 41) determines the specific localization of DLCs with their respective motor complexes. These results suggest a molecular mechanism for the specific compartmentalization of DLCs and their pro-apoptotic cargoes and implicate other protein(s) in defining the specificity between the cargoes and the DLC proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.