ArticleMolecular and cellular biology2003
Human artificial chromosomes with alpha satellite-based de novo centromeres show increased frequency of nondisjunction and anaphase lag.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
- A genomic and epigenomic view of human centromeres.Nature reviews. Genetics · 2026Review
- Protein-only centromeric chromatin assembly streamlines human artificial chromosome formation.bioRxiv : the preprint server for biology · 2026Article
- Rapid assembly of functional modules for generating human artificial chromosome constructs compatible with epigenetic centromere seeding.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Rapid assembly of functional modules for generating human artificial chromosome constructs compatible with epigenetic centromere seeding.bioRxiv : the preprint server for biology · 2025Article
- Chromosomal instability in a patient with ring chromosome 14 syndrome: a case report.Molecular cytogenetics · 2024Article
- Alpha satellite DNA biology: finding function in the recesses of the genome.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2018Review
- Linear assembly of a human centromere on the Y chromosome.Nature biotechnology · 2018Article
- Chromatin dynamics during the cell cycle at centromeres.Nature reviews. Genetics · 2017Review
- A new assay for measuring chromosome instability (CIN) and identification of drugs that elevate CIN in cancer cells.BMC cancer · 2013Article
- A new generation of human artificial chromosomes for functional genomics and gene therapy.Cellular and molecular life sciences : CMLS · 2013Review
- Sequences associated with centromere competency in the human genome.Molecular and cellular biology · 2013Article
- Functional epialleles at an endogenous human centromere.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The evolutionary life cycle of the resilient centromere.Chromosoma · 2012Review
- Neocentromeres and epigenetically inherited features of centromeres.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Review
- Article
- Phylogenetic footprinting of non-coding RNA: hammerhead ribozyme sequences in a satellite DNA family of Dolichopoda cave crickets (Orthoptera, Rhaphidophoridae).BMC evolutionary biology · 2010Article
- Prospects for the use of artificial chromosomes and minichromosome-like episomes in gene therapy.Journal of biomedicine & biotechnology · 2010Article
- Artificial chromosome formation in maize (Zea mays L.).Chromosoma · 2009Article
- HAC stability in murine cells is influenced by nuclear localization and chromatin organization.BMC cell biology · 2009Article
- An artificially constructed de novo human chromosome behaves almost identically to its natural counterpart during metaphase and anaphase in living cells.Molecular and cellular biology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human artificial chromosomes have been used to model requirements for human chromosome segregation and to explore the nature of sequences competent for centromere function. Normal human centromeres require specialized chromatin that consists of alpha satellite DNA complexed with epigenetically modified histones and centromere-specific proteins. While several types of alpha satellite DNA have been used to assemble de novo centromeres in artificial chromosome assays, the extent to which they fully recapitulate normal centromere function has not been explored. Here, we have used two kinds of alpha satellite DNA, DXZ1 (from the X chromosome) and D17Z1 (from chromosome 17), to generate human artificial chromosomes. Although artificial chromosomes are mitotically stable over many months in culture, when we examined their segregation in individual cell divisions using an anaphase assay, artificial chromosomes exhibited more segregation errors than natural human chromosomes (P < 0.001). Naturally occurring, but abnormal small ring chromosomes derived from chromosome 17 and the X chromosome also missegregate more than normal chromosomes, implicating overall chromosome size and/or structure in the fidelity of chromosome segregation. As different artificial chromosomes missegregate over a fivefold range, the data suggest that variable centromeric DNA content and/or epigenetic assembly can influence the mitotic behavior of artificial chromosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.