ArticleMolecular and cellular biology2003
PU.1 and pRB interact and cooperate to repress GATA-1 and block erythroid differentiation.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 100 citations in OpenAlex.
- PU.1 and TGF-β signaling activate the cell-surface expression of CD103 in mast cells and dendritic cells: opposite roles of GATA2 in the expression of mucosal mast cell genes.The FEBS journal · 2025Article
- GATA2 decommissioning of enhancers: A mechanism to constrain inflammatory signaling.Cell reports · 2025Article
- Article
- PU.1-c-Jun interaction is crucial for PU.1 function in myeloid development.Communications biology · 2022Article
- HDAC1 and PRC2 mediate combinatorial control in SPI1/PU.1-dependent gene repression in murine erythroleukaemia.Nucleic acids research · 2022Article
- Identification of RIOK2 as a master regulator of human blood cell development.Nature immunology · 2022Article
- GATA1 mutations in red cell disorders.IUBMB life · 2020Review
- Mechanisms of Action of Hematopoietic Transcription Factor PU.1 in Initiation of T-Cell Development.Frontiers in immunology · 2019Review
- Transcription factors, coregulators, and epigenetic marks are linearly correlated and highly redundant.PloS one · 2017Article
- E2A Antagonizes PU.1 Activity through Inhibition of DNA Binding.BioMed research international · 2016Article
- Regulation of early T-lineage gene expression and developmental progression by the progenitor cell transcription factor PU.1.Genes & development · 2015Article
- The RB tumor suppressor at the intersection of proliferation and immunity: relevance to disease immune evasion and immunotherapy.Cell cycle (Georgetown, Tex.) · 2015Review
- Article
- Drl.3 governs primitive hematopoiesis in zebrafish.Scientific reports · 2014Article
- Molecular ties between the cell cycle and differentiation in embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A Comprehensive Profile of ChIP-Seq-Based PU.1/Spi1 Target Genes in Microglia.Gene regulation and systems biology · 2014Article
- High resolution methylome analysis reveals widespread functional hypomethylation during adult human erythropoiesis.The Journal of biological chemistry · 2013Article
- H1 linker histone promotes epigenetic silencing by regulating both DNA methylation and histone H3 methylation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A network of genes antagonistic to the LIN-35 retinoblastoma protein of Caenorhabditis elegans.Genetics · 2012Article
- Concomitant inactivation of Rb and E2f8 in hematopoietic stem cells synergizes to induce severe anemia.Blood · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
PU.1 and GATA-1 are two hematopoietic specific transcription factors that play key roles in development of the myeloid and erythroid lineages, respectively. The two proteins bind to one another and inhibit each other's function in transcriptional activation and promotion of their respective differentiation programs. This mutual antagonism may be an important aspect of lineage commitment decisions. PU.1 can also act as an oncoprotein since deregulated expression of PU.1 in erythroid precursors causes erythroleukemias in mice. Studies of cultured mouse erythroleukemia cell lines indicate that one aspect of PU.1 function in erythroleukemogenesis is its ability to block erythroid differentiation by repressing GATA-1 (N. Rekhtman, F. Radparvar, T. Evans, and A. I. Skoultchi, Genes Dev. 13:1398-1411, 1999). We have investigated the mechanism of PU.1-mediated repression of GATA-1. We report here that PU.1 binds to GATA-1 on DNA. We localized the repression activity of PU.1 to a small acidic N-terminal domain that interacts with the C pocket of pRB, a well-known transcriptional corepressor. Repression of GATA-1 by PU.1 requires pRB, and pRB colocalizes with PU.1 and GATA-1 at repressed GATA-1 target genes. PU.1 and pRB also cooperate to block erythroid differentiation. Our results suggest that one of the mechanisms by which PU.1 antagonizes GATA-1 is by binding to it at GATA-1 target genes and tethering to these sites a corepressor that blocks transcriptional activity and thereby erythroid differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.