ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2003
Rapid identification of yeasts commonly found in positive blood cultures by amplification of the internal transcribed spacer regions 1 and 2.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 56 citations in OpenAlex.
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- Molecular Markers of Antifungal Resistance: Potential Uses in Routine Practice and Future Perspectives.Journal of fungi (Basel, Switzerland) · 2021Review
- Modulation of ERG Genes Expression in Clinical Isolates of Candida tropicalis Susceptible and Resistant to Fluconazole and Itraconazole.Mycopathologia · 2020Article
- Biofilm growth and IL-8 & TNF-α-inducing properties of Candida albicans in the presence of oral gram-positive and gram-negative bacteria.BMC microbiology · 2020Article
- In vitro antifungal resistance profile of Candida strains isolated from Saudi women suffering from vulvovaginitis.European journal of medical research · 2020Article
- Epidemiology and antifungal susceptibilities of yeasts causing vulvovaginitis in a teaching hospital.Mycopathologia · 2014Article
- Nosocomial bloodstream Candida infections in a tertiary-care hospital in South Brazil: a 4-year survey.Mycopathologia · 2014Article
- Candida identification: a journey from conventional to molecular methods in medical mycology.World journal of microbiology & biotechnology · 2014Review
- Rapid identification of yeasts from positive blood culture bottles by pyrosequencing.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- In vitro evaluation of putative virulence attributes of oral isolates of Candida spp. obtained from elderly healthy individuals.Mycopathologia · 2008Article
- Multiplex tandem PCR: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens.Journal of clinical microbiology · 2008Article
- Identification of medically important Candida and non-Candida yeast species by an oligonucleotide array.Journal of clinical microbiology · 2007Article
- Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions.Journal of clinical microbiology · 2006Article
- Susceptibility patterns and molecular identification of Trichosporon species.Antimicrobial agents and chemotherapy · 2005Article
- Identification of medically important molds by an oligonucleotide array.Journal of clinical microbiology · 2005Article
- Isolation of Candida famata from a patient with acute zonal occult outer retinopathy.Journal of clinical microbiology · 2005Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multiplex PCR method using one universal and eight species-specific primers was developed to rapidly identify eight yeast species found in positive blood cultures. The species-specific primers were designed from the internal transcribed spacer regions 1 and 2 of the rRNA gene, whereas the universal primer was located at the 26S rRNA gene. The eight species were Candida albicans, Candida glabrata, Candida guilliermondii, Candida krusei, Candida lusitaniae, Candida parapsilosis, Candida tropicalis, and Cryptococcus neoformans. The PCR products (116 to 630 bp) were different in length and could be effectively separated and recognized by polyacrylamide gel electrophoresis. By testing 234 positive blood cultures (237 isolates), 234 (98.7%) isolates of the above eight species were correctly identified by the multiplex PCR. The present method is simple to perform and can be completed within 6 h.
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