ArticleJournal of virology2003
Murine leukemia virus particle assembly quantitated by fluorescence microscopy: role of Gag-Gag interactions and membrane association.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Determinants in the HTLV-1 Capsid Major Homology Region that are Critical for Virus Particle Assembly.Journal of molecular biology · 2024Article
- Intact Viral Particle Counts Measured by Flow Virometry Provide Insight into the Infectivity and Genome Packaging Efficiency of Moloney Murine Leukemia Virus.Journal of virology · 2020Article
- Adenovirus based virus-like-vaccines targeting endogenous retroviruses can eliminate growing colorectal cancers in mice.Oncotarget · 2019Article
- ESCRT Requirements for Murine Leukemia Virus Release.Viruses · 2016Article
- Macropinocytosis is the entry mechanism of amphotropic murine leukemia virus.Journal of virology · 2015Article
- HIV-1 assembly, budding, and maturation.Cold Spring Harbor perspectives in medicine · 2012Review
- Synchronized retrovirus fusion in cells expressing alternative receptor isoforms releases the viral core into distinct sub-cellular compartments.PLoS pathogens · 2012Article
- Inhibition of HIV-1 endocytosis allows lipid mixing at the plasma membrane, but not complete fusion.Retrovirology · 2011Article
- Protein transduction from retroviral Gag precursors.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Targeting of murine leukemia virus gag to the plasma membrane is mediated by PI(4,5)P2/PS and a polybasic region in the matrix.Journal of virology · 2010Article
- Gamma-retroviral vectors enveloped with an antibody and an engineered fusogenic protein achieved antigen-specific targeting.Biotechnology and bioengineering · 2008Article
- Dominant negative inhibition of human immunodeficiency virus particle production by the nonmyristoylated form of gag.Journal of virology · 2008Article
- Intermolecular interactions between retroviral Gag proteins in the nucleus.Journal of virology · 2008Article
- TR1.3 viral pathogenesis and syncytium formation are linked to Env-Gag cooperation.Journal of virology · 2007Article
- Matrix fibronectin binds gammaretrovirus and assists in entry: new light on viral infections.Journal of virology · 2007Article
- Caveolin-1 interacts with the Gag precursor of murine leukaemia virus and modulates virus production.Virology journal · 2006Article
- Amphotropic murine leukemia virus is preferentially attached to cholesterol-rich microdomains after binding to mouse fibroblasts.Virology journal · 2006Article
- Time-resolved imaging of HIV-1 Env-mediated lipid and content mixing between a single virion and cell membrane.Molecular biology of the cell · 2005Article
- The retroviral capsid domain dictates virion size, morphology, and coassembly of gag into virus-like particles.Journal of virology · 2005Article
- Caveola-dependent endocytic entry of amphotropic murine leukemia virus.Journal of virology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to track the assembly of murine leukemia virus (MLV), we used fluorescence microscopy to visualize particles containing Gag molecules fused to fluorescent proteins (FPs). Gag-FP chimeras budded from cells to produce fluorescent spots, which passed through the same pore-size filters and sedimented at the same velocity as authentic MLV. N-terminal myristylation of Gag-FPs was necessary for particle formation unless wild-type Gag was coexpressed. By labeling nonmyristylated Gag with yellow FP and wild-type Gag with cyan FP, we could quantitate the coincorporation of two proteins into single particles. This experiment showed that nonmyristylated Gag was incorporated into mixed particles at approximately 50% the efficiency of wild-type Gag. Mutations that inhibit Gag-Gag interactions (K. Alin and S. P. Goff, Virology 216:418-424, 1996; K. Alin and S. P. Goff, Virology 222:339-351, 1996) were then introduced into the capsid (CA) region of Gag-FPs. The mutations P150L and R119C/P133L inhibited fluorescent particle formation by these Gag-FPs, but Gag-FPs containing these mutations could be efficiently incorporated into particles when coexpressed with wild-type Gag. When these mutations were introduced into nonmyristylated Gag-FPs, no incorporation into particles in the presence of wild-type Gag was detected. These data suggest that two independent mechanisms, CA interactions and membrane association following myristylation, cooperate in MLV Gag assembly and budding.
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