ArticleJournal of virology2003
Protein products of the open reading frames encoding nonstructural proteins of human astrovirus serotype 8.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 58 citations in OpenAlex.
- Viral protease-mediated polyprotein processing in human astroviruses.Npj viruses · 2026Article
- Identification of viral protease-dependent cleavage sites within the human astrovirus polyprotein.Journal of virology · 2025Article
- Impact of Wastewater Use for Irrigation and Contamination of Lettuce by Enteric Viruses: Case of Ouagadougou Market Gardening Sites, Burkina Faso.Food and environmental virology · 2024Article
- The Association of Human Astrovirus with Extracellular Vesicles Facilitates Cell Infection and Protects the Virus from Neutralizing Antibodies.Journal of virology · 2022Article
- Article
- The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3.Viruses · 2021Article
- Article
- The Ubiquitin-Proteasome System Is Necessary for Efficient Replication of Human Astrovirus.Journal of virology · 2018Article
- Structural, Mechanistic, and Antigenic Characterization of the Human Astrovirus Capsid.Journal of virology · 2015Article
- Identification of Host Cell Factors Associated with Astrovirus Replication in Caco-2 Cells.Journal of virology · 2015Article
- Human astroviruses.Clinical microbiology reviews · 2014Review
- Characterization of human astrovirus cell entry.Journal of virology · 2014Article
- PTB binds to the 3' untranslated region of the human astrovirus type 8: a possible role in viral replication.PloS one · 2014Article
- Article
- Crystal structure of the human astrovirus capsid spike.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The C-terminal nsP1a protein of human astrovirus is a phosphoprotein that interacts with the viral polymerase.Journal of virology · 2011Article
- Association of the astrovirus structural protein VP90 with membranes plays a role in virus morphogenesis.Journal of virology · 2007Article
- Different rates of (non-)synonymous mutations in astrovirus genes; correlation with gene function.Virology journal · 2007Article
- Identification of structural domains involved in astrovirus capsid biology.Viral immunology · 2005Review
- C-terminal nsP1a protein of human astrovirus colocalizes with the endoplasmic reticulum and viral RNA.Journal of virology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human astroviruses have a positive-strand RNA genome, which contains three open reading frames (ORF1a, ORF1b, and ORF2). The genomic RNA is translated into two nonstructural polyproteins, nsp1a and nsp1ab, that contain sequences derived from ORF1a and from both ORF1a and ORF1b, respectively. Proteins nsp1a and nsp1ab are thought to be proteolytically processed to yield the viral proteins implicated in the replication of the virus genome; however, the intermediate and final products of this processing have been poorly characterized. To identify the cleavage products of the nonstructural polyproteins of a human astrovirus serotype 8 strain, antisera to selected recombinant proteins were produced and were used to analyze the viral proteins synthesized in astrovirus-infected Caco-2 cells and in cells transfected with recombinant plasmids expressing the ORF1a and ORF1b polyproteins. Pulse-chase experiments identified proteins of approximately 145, 88, 85, and 75 kDa as cleavage intermediates during the polyprotein processing. In addition, these experiments and kinetic analysis of the synthesis of the viral proteins identified polypeptides of 57, 20, and 19 kDa, as well as two products of around 27 kDa, as final cleavage products, with the 57-kDa polypeptide most probably being the virus RNA polymerase and the two approximately 27-kDa products being the viral protease. Based on the differential reactivities of the astrovirus proteins with the various antisera used, the individual polypeptides detected were mapped to the virus ORF1a and ORF1b regions.
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