ArticleThe Journal of cell biology2003
How VASP enhances actin-based motility.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 150 citations in OpenAlex.
- Zyxin is important for the stability and function of podocytes, especially during mechanical stretch.Communications biology · 2024Article
- Ena/VASP clustering at microspike tips involves lamellipodin but not I-BAR proteins, and absolutely requires unconventional myosin-X.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Review
- Fe65: A Scaffolding Protein of Actin Regulators.Cells · 2021Review
- Intra-bundle contractions enable extensile properties of active actin networks.Scientific reports · 2021Article
- Capping protein is dispensable for polarized actin network growth and actin-based motility.The Journal of biological chemistry · 2020Article
- Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion.eLife · 2020Article
- Efficiency of lamellipodia protrusion is determined by the extent of cytosolic actin assembly.Molecular biology of the cell · 2017Article
- Virulent Burkholderia species mimic host actin polymerases to drive actin-based motility.Cell · 2015Article
- Reconstitution of actin-based motility by vasodilator-stimulated phosphoprotein (VASP) depends on the recruitment of F-actin seeds from the solution produced by cofilin.The Journal of biological chemistry · 2014Article
- Control of actin-based motility through localized actin binding.Physical biology · 2013Article
- CDC42 switches IRSp53 from inhibition of actin growth to elongation by clustering of VASP.The EMBO journal · 2013Article
- Multiple actin binding domains of Ena/VASP proteins determine actin network stiffening.European biophysics journal : EBJ · 2012Article
- Filopodium retraction is controlled by adhesion to its tip.Journal of cell science · 2012Article
- Actin network growth under load.Biophysical journal · 2012Article
- Actin dynamics associated with focal adhesions.International journal of cell biology · 2012Article
- The Eps8/IRSp53/VASP network differentially controls actin capping and bundling in filopodia formation.PLoS computational biology · 2011Article
- Molecular mechanism of Ena/VASP-mediated actin-filament elongation.The EMBO journal · 2011Article
- Modeling capping protein FRAP and CALI experiments reveals in vivo regulation of actin dynamics.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Dendritic actin filament nucleation causes traveling waves and patches.Physical review letters · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The function of vasodilator-stimulated phosphoprotein (VASP) in motility is analyzed using a biomimetic motility assay in which ActA-coated microspheres propel themselves in a medium containing actin, the Arp2/3 complex, and three regulatory proteins in the absence or presence of VASP. Propulsion is linked to cycles of filament barbed end attachment-branching-detachment-growth in which the ActA-activated Arp2/3 complex incorporates at the junctions of branched filaments. VASP increases the velocity of beads. VASP increases branch spacing of filaments in the actin tail, as it does in lamellipodia in living cells. The effect of VASP on branch spacing of Arp2/3-induced branched actin arrays is opposed to the effect of capping proteins. However, VASP does not compete with capping proteins for binding barbed ends of actin filaments. VASP enhances branched actin polymerization only when ActA is immobilized on beads or on Listeria. VASP increases the rate of dissociation of the branch junction from immobilized ActA, which is the rate-limiting step in the catalytic cycle of site-directed filament branching.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.