ArticleThe Biochemical journal2004
N-Formyl peptide receptor subtypes in human neutrophils activate L-plastin phosphorylation through different signal transduction intermediates.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 35 citations in OpenAlex.
- Polymorphonuclear Cell Chemotaxis and Suicidal NETosis: Simultaneous Observation Using fMLP, PMA, H7, and Live Cell Imaging.Journal of immunology research · 2020Article
- LRRK1 regulation of actin assembly in osteoclasts involves serine 5 phosphorylation of L-plastin.Journal of cellular biochemistry · 2018Article
- The actin-bundling protein L-plastin supports T-cell motility and activation.Immunological reviews · 2013Review
- The actin-bundling protein L-plastin: a critical regulator of immune cell function.International journal of cell biology · 2012Article
- Priming of eosinophils by GM-CSF is mediated by protein kinase CbetaII-phosphorylated L-plastin.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Subcellular localisation of the p40phox component of NADPH oxidase involves direct interactions between the Phox homology domain and F-actin.The international journal of biochemistry & cell biology · 2010Article
- Quantitative kinetic study of the actin-bundling protein L-plastin and of its impact on actin turn-over.PloS one · 2010Article
- Exploiting the Annexin A1 pathway for the development of novel anti-inflammatory therapeutics.British journal of pharmacology · 2009Review
- Biology and therapeutic potential of cannabinoid CB2 receptor inverse agonists.British journal of pharmacology · 2008Review
- Membrane tubulovesicular extensions (cytonemes): secretory and adhesive cellular organelles.Cell adhesion & migrationReview
- Microbial alkaloid staurosporine induces formation of nanometer-wide membrane tubular extensions (cytonemes, membrane tethers) in human neutrophils.Cell adhesion & migrationArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the coupling of the fMLP (N -formyl-L-methionyl-L-leucyl-L-phenylalanine; 'chemotactic peptide') receptor with phosphorylation of the actin-binding protein L-plastin in neutrophils. Using two-dimensional IEF (isoelectric focusing)/PAGE and MALDI-TOF (matrix-assisted laser desorption ionization-time-of-flight)-MS, L-plastin was identified as a major phosphoprotein in fMLP-stimulated neutrophils whose phosphorylation was dependent on phosphoinositide 3-kinase, PLD (phospholipase D) and PKC (protein kinase C) activity. Two fMLP receptor subtypes were identified in neutrophils, characterized by a distinct sensitivity to fMLP and antagonistic peptides. Both receptor subtypes induced the phosphorylation of L-plastin. L-plastin phosphorylation induced by low-affinity fMLP receptors involves an action of phosphoinositide 3-kinase, PLD and PKC isotypes. In contrast, none of these intermediates are utilized by high-affinity fMLP receptors in the phosphorylation of L-plastin. However, the PKC inhibitor Ro-31-8220 inhibits L-plastin phosphorylation induced by the high-affinity fMLP receptor. Thus, an as yet unknown Ro-31-8220-sensitive kinase regulates L-plastin phosphorylation in response to the high-affinity fMLP receptor. The results suggest a model in which receptor subtypes induce a similar endpoint event through different signal-transduction intermediates. This may be relevant in the context of cell migration in which one receptor subpopulation may become desensitized in a concentration gradient of chemoattractant.
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