ArticleThe Plant cell2003
Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity.
Article in The Plant cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 588 papers, 3 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
588 citing papers in PubMed, 3 syntheses or guidelines pooled it, 1,498 citations in OpenAlex.
- Meta-analysis of seed weight QTLome using a consensus and highly dense genetic map in Brassica napus L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Pooled it
- Role of cytokinins in seed development in pulses and oilseed crops: Current status and future perspective.Frontiers in genetics · 2022Pooled it
- Chickpea Biofortification for Cytokinin DehydrogenaseFrontiers in genetics · 2022Pooled it
- Mutation of SCARECROW-LIKE28 or CYTOKININ OXIDASE3/5 suppresses rosette branching in Arabidopsis.Journal of experimental botany · 2026Article
- Article
- Natural variation in OsMYB305 downregulating cytokinin-mediated inhibition of leaf senescence contributes to regional adaptation in rice.The New phytologist · 2026Article
- Arbuscular Mycorrhizal Fungus System Recruits Sphingomonas spp. to Enhance Drought Resistance in Peanut.Plant, cell & environment · 2026Article
- Cytokinin receptor AHK3 influences leaf size by modulating trans-zeatin-type cytokinin levels in xylem.Plant & cell physiology · 2026Article
- Article
- Targeted metabolomic and gene expression analysis reveals an organ-specific response to chronic ionizing radiation exposure during Pinus sylvestris seedling development.BMC plant biology · 2026Article
- Fine mapping and transcriptomic analysis reveal BnaMMS, a novel locus regulating multi-main-stem phenotype via auxin pathway in rapeseed.BMC plant biology · 2026Article
- Overexpression of an Alfalfa Gene,Genes · 2026Article
- Exogenous Hormone Treatments Reveal Species-Specific Regulation of Individual Components of Root Architecture and Salt Ion Accumulation in Cultivated and Wild Tomatoes.Plant direct · 2026Article
- Auxin-cytokinin crosstalk and differential growth: complex relationships driving carpel initiation.Journal of experimental botany · 2026Article
- ABT Promotes Adventitious Root Formation in Mulberry Cuttings by Coordinating Hormonal Homeostasis and Defense Priming.Current issues in molecular biology · 2026Article
- Root-specific cytokinin catabolism promotes Nicotiana root growth and resilience to drought stress.Planta · 2026Article
- Differential activity of CYTOKININ OXIDASE/DEHYDROGENASE3 (CKX3) and CKX5 genes in regulating yield components in Brassica napus L.Plant molecular biology · 2026Article
- Evolutionary adaptations to the hormonal regulation of vascular tissue development.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Effect of Light and Cytokinin Modulators on Adventitious Shooting inPlants (Basel, Switzerland) · 2026Article
- Cytokinin availability and chloroplast functional lifetime in senescing leaves.Frontiers in plant science · 2026Review
528 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinins are hormones that regulate cell division and development. As a result of a lack of specific mutants and biochemical tools, it has not been possible to study the consequences of cytokinin deficiency. Cytokinin-deficient plants are expected to yield information about processes in which cytokinins are limiting and that, therefore, they might regulate. We have engineered transgenic Arabidopsis plants that overexpress individually six different members of the cytokinin oxidase/dehydrogenase (AtCKX) gene family and have undertaken a detailed phenotypic analysis. Transgenic plants had increased cytokinin breakdown (30 to 45% of wild-type cytokinin content) and reduced expression of the cytokinin reporter gene ARR5:GUS (beta-glucuronidase). Cytokinin deficiency resulted in diminished activity of the vegetative and floral shoot apical meristems and leaf primordia, indicating an absolute requirement for the hormone. By contrast, cytokinins are negative regulators of root growth and lateral root formation. We show that the increased growth of the primary root is linked to an enhanced meristematic cell number, suggesting that cytokinins control the exit of cells from the root meristem. Different AtCKX-green fluorescent protein fusion proteins were localized to the vacuoles or the endoplasmic reticulum and possibly to the extracellular space, indicating that subcellular compartmentation plays an important role in cytokinin biology. Analyses of promoter:GUS fusion genes showed differential expression of AtCKX genes during plant development, the activity being confined predominantly to zones of active growth. Our results are consistent with the hypothesis that cytokinins have central, but opposite, regulatory functions in root and shoot meristems and indicate that a fine-tuned control of catabolism plays an important role in ensuring the proper regulation of cytokinin functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.