ArticleEukaryotic cell2003
Fission yeast Sap1 protein is essential for chromosome stability.
Article in Eukaryotic cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- The Fission Yeast Mating-Type Switching Motto: "One-for-Two" and "Two-for-One".Microbiology and molecular biology reviews : MMBR · 2023Review
- Mating-type switching by homology-directed recombinational repair: a matter of choice.Current genetics · 2019Review
- Molecular signature of the imprintosome complex at the mating-type locus in fission yeast.Microbial cell (Graz, Austria) · 2018Article
- Shelterin components mediate genome reorganization in response to replication stress.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Review
- Review
- Arrested replication forks guide retrotransposon integration.Science (New York, N.Y.) · 2015Article
- The Long Terminal Repeat Retrotransposons Tf1 and Tf2 of Schizosaccharomyces pombe.Microbiology spectrum · 2015Review
- Implementation of the CRISPR-Cas9 system in fission yeast.Nature communications · 2014Article
- DNA polymerase α (swi7) and the flap endonuclease Fen1 (rad2) act together in the S-phase alkylation damage response in S. pombe.PloS one · 2012Article
- Article
- Article
- Molecular architecture of a eukaryotic DNA replication terminus-terminator protein complex.Molecular and cellular biology · 2006Article
- The mating type switch-activating protein Sap1 Is required for replication fork arrest at the rRNA genes of fission yeast.Molecular and cellular biology · 2005Article
- Molecular and cellular dissection of mating-type switching steps in Schizosaccharomyces pombe.Molecular and cellular biology · 2005Article
- Biochemical interactions between proteins and mat1 cis-acting sequences required for imprinting in fission yeast.Molecular and cellular biology · 2004Article
- Role of the fission yeast SUMO E3 ligase Pli1p in centromere and telomere maintenance.The EMBO journal · 2004Article
- Formation, maintenance and consequences of the imprint at the mating-type locus in fission yeast.The EMBO journal · 2004Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sap1 is a dimeric sequence-specific DNA binding-protein, initially identified for its role in mating-type switching of the fission yeast Schizosaccharomyces pombe. The protein is relatively abundant, around 10,000 dimers/cell, and is localized in the nucleus. sap1+ is essential for viability, and transient overexpression is accompanied by rapid cell death, without an apparent checkpoint response and independently of mating-type switching. Time lapse video microscopy of living cells revealed that the loss of viability is accompanied by abnormal mitosis and chromosome fragmentation. Overexpression of the C terminus of Sap1 induces minichromosome loss associated with the "cut" phenotype (uncoupling mitosis and cytokinesis). These phenotypes are favored when the C terminus of Sap1 is overexpressed during DNA replication. Fluorescence in situ hybridization experiments demonstrated that the cut phenotype is related to precocious centromere separation, a typical marker for loss of cohesion. We propose that Sap1 is an architectural chromatin-associated protein, required for chromosome organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.