ArticleEukaryotic cell2003
Chitin synthesis in Saccharomyces cerevisiae in response to supplementation of growth medium with glucosamine and cell wall stress.
Article in Eukaryotic cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 163 citations in OpenAlex.
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- Accumulation of Trehalose 6-Phosphate in Candidozyma auris results in Decreased Echinocandin Resistance and Tolerance.Nature communications · 2025Article
- The phenomenon of anhydrobiosis-structural and functional changes in yeast cells.Applied microbiology and biotechnology · 2025Review
- Enhancing Chitin Production as a Fermentation Byproduct through a Genetic Toolbox That Activates the Cell Wall Integrity Response.ACS synthetic biology · 2025Article
- "You Are What You Eat": How Fungal Adaptation Can Be Leveraged toward Myco-Material Properties.Global challenges (Hoboken, NJ) · 2024Article
- Sugar Utilization-Associated Food-Grade Selection Markers in Lactic Acid Bacteria and Yeast.Polish journal of microbiology · 2024Review
- Fluorescence and Biochemical Assessment of the Chitin and Chitosan Content of Cryptococcus.Methods in molecular biology (Clifton, N.J.) · 2024Article
- CSE-8, a filamentous fungus-specific Shr3-like chaperone, facilitates endoplasmic reticulum exit of chitin synthase CHS-3 (class I) inFrontiers in fungal biology · 2024Article
- Linear and Nonlinear Regression Analysis for the Adsorption of Remazol Dye by Romanian Brewery Waste By-Product,International journal of molecular sciences · 2022Article
- The Mnn10/Anp1-dependent N-linked outer chain glycan is dispensable for Candida albicans cell wall integrity.Genetics · 2022Article
- Slt2 Is Required to Activate ER-Stress-Protective Mechanisms through TORC1 Inhibition and Hexosamine Pathway Activation.Journal of fungi (Basel, Switzerland) · 2022Article
- Production of galactosylated complex-type N-glycans in glycoengineered Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2022Article
- SsAGM1-Mediated Uridine Diphosphate-Frontiers in microbiology · 2022Article
- Class I myosin mediated endocytosis and polarization growth is essential for pathogenicity of Magnaporthe oryzae.Applied microbiology and biotechnology · 2021Article
- Morphological and metabolic changes in an aged strain of Agaricus bisporus As2796.Applied microbiology and biotechnology · 2021Article
- Rapid screening method of Saccharomyces cerevisiae mutants using calcofluor white and aniline blue.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- With an Ear Up against the Wall: An Update on Mechanoperception inPlants (Basel, Switzerland) · 2021Review
- Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis inThe plant pathology journal · 2020Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
In Saccharomyces cerevisiae most chitin is synthesized by Chs3p, which deposits chitin in the lateral cell wall and in the bud-neck region during cell division. We have recently found that addition of glucosamine (GlcN) to the growth medium leads to a three- to fourfold increase in cell wall chitin levels. We compared this result to the increases in cellular chitin levels associated with cell wall stress and with treatment of yeast with mating pheromone. Since all three phenomena lead to increases in precursors of chitin, we hypothesized that chitin synthesis is at least in part directly regulated by the size of this pool. This hypothesis was strengthened by our finding that addition of GlcN to the growth medium causes a rapid increase in chitin synthesis without any pronounced change in the expression of more than 6,000 genes monitored with Affymetrix gene expression chips. In other studies we found that the specific activity of Chs3p is higher in the total membrane fractions from cells grown in GlcN and from mutants with weakened cell walls. Sucrose gradient analysis shows that Chs3p is present in an inactive form in what may be Golgi compartments but as an active enzyme in other intracellular membrane-bound vesicles, as well as in the plasma membrane. We conclude that Chs3p-dependent chitin synthesis in S. cerevisiae is regulated both by the levels of intermediates of the UDP-GlcNAc biosynthetic pathway and by an increase in the activity of the enzyme in the plasma membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.