ArticleEukaryotic cell2003
D-xylose metabolism in Hypocrea jecorina: loss of the xylitol dehydrogenase step can be partially compensated for by lad1-encoded L-arabinitol-4-dehydrogenase.
Article in Eukaryotic cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 65 citations in OpenAlex.
- Barcoded mutant library enables high-throughput functional genomics in a filamentous fungus.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The genome-scale sugar metabolic model fromCurrent research in microbial sciences · 2026Article
- Non-canonical D-xylose and L-arabinose metabolism via D-arabitol in the oleaginous yeast Rhodosporidium toruloides.Microbial cell factories · 2023Article
- Revisiting a 'simple' fungal metabolic pathway reveals redundancy, complexity and diversity.Microbial biotechnology · 2021Article
- Screening and Growth Characterization of Non-conventional Yeasts in a Hemicellulosic Hydrolysate.Frontiers in bioengineering and biotechnology · 2021Article
- Xylose Metabolism and the Effect of Oxidative Stress on Lipid and Carotenoid Production inFrontiers in bioengineering and biotechnology · 2020Article
- Targets of light signalling in Trichoderma reesei.BMC genomics · 2013Article
- A novel L-xylulose reductase essential for L-arabinose catabolism in Trichoderma reesei.Biochemistry · 2013Article
- Xylanase gene transcription in Trichoderma reesei is triggered by different inducers representing different hemicellulosic pentose polymers.Eukaryotic cell · 2013Article
- L-xylo-3-hexulose reductase is the missing link in the oxidoreductive pathway for D-galactose catabolism in filamentous fungi.The Journal of biological chemistry · 2012Article
- Deciphering transcriptional regulatory mechanisms associated with hemicellulose degradation in Neurospora crassa.Eukaryotic cell · 2012Article
- Correlation of gene expression and protein production rate - a system wide study.BMC genomics · 2011Article
- L-arabitol is the actual inducer of xylanase expression in Hypocrea jecorina (Trichoderma reesei).Applied and environmental microbiology · 2011Article
- Analysis of regulation of pentose utilisation in Aspergillus niger reveals evolutionary adaptations in Eurotiales.Studies in mycology · 2011Article
- Fungal arabinan and L-arabinose metabolism.Applied microbiology and biotechnology · 2011Review
- Identification of an L-arabinose reductase gene in Aspergillus niger and its role in L-arabinose catabolism.The Journal of biological chemistry · 2010Article
- Review: Global nutrient profiling by Phenotype MicroArrays: a tool complementing genomic and proteomic studies in conidial fungi.Journal of Zhejiang University. Science. B · 2010Review
- Molecular regulation of arabinan and L-arabinose metabolism in Hypocrea jecorina (Trichoderma reesei).Eukaryotic cell · 2009Article
- Metabolic engineering strategies for the improvement of cellulase production by Hypocrea jecorina.Biotechnology for biofuels · 2009Article
- A single amino acid change (Y318F) in the L-arabitol dehydrogenase (LadA) from Aspergillus niger results in a significant increase in affinity for D-sorbitol.BMC microbiology · 2009Article
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Abstract
With the goal of the genetic characterization of the D-xylose pathway in Hypocrea jecorina (anamorph: Trichoderma reesei), we cloned the xdh1 gene, encoding NAD-xylitol dehydrogenase, which catalyzes the second step of fungal D-xylose catabolism. This gene encodes a 363-amino-acid protein which has a mass of 38 kDa, belongs to the zinc-containing alcohol dehydrogenase family, exhibits high sequence identity to the published sequences of xylitol dehydrogenases from yeast origins, but contains a second, additional binding site for Zn2+. The enzyme catalyzed the NAD-dependent oxidation of xylitol and D-sorbitol and the NADH-dependent reduction of D-xylulose and D-fructose. No activity was observed with NADP, L-arabinose, or L-arabinitol. A single 1.4-kb transcript was formed during growth on xylan, D-xylose, L-arabinose, L-arabinitol and, at a lower abundance, xylitol, D-galactose, galactitol, and lactose but not on D-glucose and glycerol. xdh1 deletion mutants exhibited 50% reduced growth rates on D-xylose, whereas growth rates on xylitol remained unaltered. These mutants contained 30% of the xylitol dehydrogenase activity of the parent strain, indicating the presence of a second xylitol dehydrogenase. This activity was shown to be due to lad1-encoded L-arabinitol-4-dehydrogenase, because H. jecorina xdh1 lad1 double-deletion strains failed to grow on D-xylose or xylitol. In contrast, lad1 deletion strains of H. jecorina grew normally on these carbon sources. These results show that H. jecorina contains a single xylitol dehydrogenase which is encoded by xdh1 and is involved in the metabolism of D-xylose and that lad1-encoded L-arabinitol-4-dehydrogenase can compensate for it partially in mutants with a loss of xdh1 function.
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