ArticlePlant physiology2003
Epigenetic switch from posttranscriptional to transcriptional silencing is correlated with promoter hypermethylation.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 80 citations in OpenAlex.
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- Genome-wide high-resolution mapping of DNA methylation reveals epigenetic variation in the offspring of sexual and asexual propagation in Robinia pseudoacacia.Plant cell reports · 2021Article
- Detailed insight into the dynamics of the initial phases of de novo RNA-directed DNA methylation in plant cells.Epigenetics & chromatin · 2019Article
- 5-Azacytidine mediated reactivation of silenced transgenes in potato (Solanum tuberosum) at the whole plant level.Plant cell reports · 2017Article
- Effect of orientation of transcription of a gene in an inverted transferred DNA repeat on transcriptional gene silencing in rice transgenics-a case study.Physiology and molecular biology of plants : an international journal of functional plant biology · 2015Article
- Reconstructing de novo silencing of an active plant retrotransposon.Nature genetics · 2013Article
- Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture.Epigenetics · 2013Article
- An evolutionary view of plant tissue culture: somaclonal variation and selection.Plant cell reports · 2012Review
- In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning.Journal of experimental botany · 2012Article
- Paramutation of tobacco transgenes by small RNA-mediated transcriptional gene silencing.Epigenetics · 2011Article
- Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation.Annals of botany · 2010Article
- Cloning of transgenic tobacco BY-2 cells; an efficient method to analyse and reduce high natural heterogeneity of transgene expression.BMC plant biology · 2009Article
- Cell culture-induced gradual and frequent epigenetic reprogramming of invertedly repeated tobacco transgene epialleles.Plant physiology · 2009Article
- Developmentally early and late onset of Rps10 silencing in Arabidopsis thaliana: genetic and environmental regulation.Journal of experimental botany · 2009Article
- Development of IFN-gamma resistance is associated with attenuation of SOCS genes induction and constitutive expression of SOCS 3 in melanoma cells.British journal of cancer · 2007Article
- Transgenic cassava resistance to African cassava mosaic virus is enhanced by viral DNA-A bidirectional promoter-derived siRNAs.Plant molecular biology · 2007Article
- A transcriptome-based characterization of habituation in plant tissue culture.Plant physiology · 2006Article
- The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco.Nucleic acids research · 2006Article
- Characteristics of the tomato nuclear genome as determined by sequencing undermethylated EcoRI digested fragments.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Dedifferentiation of tobacco cells is associated with ribosomal RNA gene hypomethylation, increased transcription, and chromatin alterations.Plant physiology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Changes in the distribution of methylcytosine residues along a transgene locus of tobacco (Nicotiana tabacum) in relation to the type of gene silencing were studied in parental plant leaves, calli, and regenerated plants derived thereof. Parental-silenced HeLo1 (hemizygous for locus 1) plants show posttranscriptional silencing of the residing nptII (neomycin phosphotransferase II) transgene and cytosine methylation restricted to the 3' end and center part of the transcribed region. Here, we report that with an increasing number of cell cycles, DNA methylation changes gradually, and methylation is introduced into the promoter during cell culture and more slowly in vegetatively propagated plants. After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical (CG and CNG) sites was almost complete within the 5' end of the nptII-transcribed region and the 35S promoter. Further, methylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of methylation was significantly reduced in the 3' end of the transcribed region when compared with locus 1. The newly established epigenetic patterns were stably transmitted from calli into regenerated plants and their progeny. The protein and steady-state RNA levels remained low in locus 1E, whereas with nuclear run-on assays, no detectable amounts of primary transcripts were found along the nptII gene, indicating that the methylated promoter became inactivated. The results suggest that a switch between posttranscriptional and transcriptional gene silencing could be a mechanism leading to irrevocable shut down of gene expression within a finite number of generations.
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