ArticlePlant physiology2003
PRT1 of Arabidopsis is a ubiquitin protein ligase of the plant N-end rule pathway with specificity for aromatic amino-terminal residues.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 82 citations in OpenAlex.
- Functional Divergence of the Arg/N-Degron Pathway Between the CropPlant direct · 2026Article
- Structural basis for the recognition and ubiquitylation of type-2 N-degron substrate by PRT1 plant N-recognin.Nature communications · 2025Article
- Ubiquitin E3 ligases in the plant Arg/N-degron pathway.The Biochemical journal · 2024Review
- The SMXL8-AGL9 module mediates crosstalk between strigolactone and gibberellin to regulate strigolactone-induced anthocyanin biosynthesis in apple.The Plant cell · 2024Article
- The lowdown on breakdown: Open questions in plant proteolysis.The Plant cell · 2024Review
- BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition.The Plant cell · 2024Article
- P-NADs:Heliyon · 2024Article
- Protocols for Studying Protein Stability in an Arabidopsis Protoplast Transient Expression System.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A Yeast-Based Functional Assay to Study Plant N-Degron - N-Recognin Interactions.Frontiers in plant science · 2021Article
- A simple and efficientPlant direct · 2020Article
- ThePlant direct · 2019Article
- Differential N-end Rule Degradation of RIN4/NOI Fragments Generated by the AvrRpt2 Effector Protease.Plant physiology · 2019Article
- TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity.Nature communications · 2019Article
- Tandem Fluorescent Protein Timers for Noninvasive Relative Protein Lifetime Measurement in Plants.Plant physiology · 2019Article
- Modulating Protein Stability to Switch Toxic Protein Function On and Off in Living Cells.Plant physiology · 2019Article
- Genetic interactions between ABA signalling and the Arg/N-end rule pathway during Arabidopsis seedling establishment.Scientific reports · 2018Article
- Insights into degradation mechanism of N-end rule substrates by p62/SQSTM1 autophagy adapter.Nature communications · 2018Article
- N-terminomics reveals control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway.The New phytologist · 2018Article
- Real-time detection of N-end rule-mediated ubiquitination via fluorescently labeled substrate probes.The New phytologist · 2018Article
- Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation inGenes & development · 2017Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene PRT1 of Arabidopsis, encoding a 45-kD protein with two RING finger domains, is essential for the degradation of F-dihydrofolate reductase, a model substrate of the N-end rule pathway of protein degradation. We have determined the function of PRT1 by expression in yeast (Saccharomyces cerevisiae). PRT1 can act as a ubiquitin protein ligase in the heterologous host. The identified substrates of PRT1 have an aromatic residue at their amino-terminus, indicating that PRT1 mediates degradation of N-end rule substrates with aromatic termini but not of those with aliphatic or basic amino-termini. Expression of model substrates in mutant and wild-type plants confirmed this substrate specificity. A ligase activity exclusively devoted to aromatic amino-termini of the N-end rule pathway is apparently unique to plants. The results presented also imply that other known substrates of the plant N-end rule pathway are ubiquitylated by one or more different ubiquitin protein ligases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.