ArticlePlant physiology2003
Site-specific integration of Agrobacterium tumefaciens T-DNA via double-stranded intermediates.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
64 citing papers in PubMed, 146 citations in OpenAlex.
- Arabidopsis DNA repair mutants can integrate Agrobacterium T-DNA into the plant genome.The New phytologist · 2026Article
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Versatile Applications of CRISPR-Based Programmable T-DNA Integration in Plants.Plant biotechnology journal · 2025Article
- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- Agrobacterium-Mediated Transformation for the Development of Transgenic Crops; Present and Future Prospects.Molecular biotechnology · 2024Review
- Genetic dissection of mutagenic repair and T-DNA capture at CRISPR-induced DNA breaks inPNAS nexus · 2024Article
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- Identification of T-DNA structure and insertion site in transgenic crops using targeted capture sequencing.Frontiers in plant science · 2023Article
- Genetic modification, intercellular communication, and epigenetic regulation in plants: An outlook.Biochemical and biophysical research communications · 2022Review
- Distinct mechanisms for genomic attachment of the 5' and 3' ends of Agrobacterium T-DNA in plants.Nature plants · 2022Article
- Review
- I-SceI and customized meganucleases-mediated genome editing in tomato and oilseed rape.Transgenic research · 2022Article
- Improvements of nuclease and nickase gene modification techniques for the treatment of genetic diseases.Frontiers in genome editing · 2022Review
- Plant DNA Repair andInternational journal of molecular sciences · 2021Review
- Sequence modification on demand: search and replace tools for precise gene editing in plants.Transgenic research · 2021Review
- Targeted DNA insertion in plants.Proceedings of the National Academy of Sciences of the United States of America · 2021Review
- Modulation of plant DNA damage response gene expression during Agrobacterium infection.Biochemical and biophysical research communications · 2021Article
- Article
- Article
- Integration, abundance, and transmission of mutations and transgenes in a series of CRISPR/Cas9 soybean lines.BMC biotechnology · 2020Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens-mediated genetic transformation involves transfer of a single-stranded T-DNA molecule (T strand) into the host cell, followed by its integration into the plant genome. The molecular mechanism of T-DNA integration, the culmination point of the entire transformation process, remains largely obscure. Here, we studied the roles of double-stranded breaks (DSBs) and double-stranded T-DNA intermediates in the integration process. We produced transgenic tobacco (Nicotiana tabacum) plants carrying an I-SceI endonuclease recognition site that, upon cleavage with I-SceI, generates DSB. Then, we retransformed these plants with two A. tumefaciens strains: one that allows transient expression of I-SceI to induce DSB and the other that carries a T-DNA with the I-SceI site and an integration selection marker. Integration of this latter T-DNA as full-length and I-SceI-digested molecules into the DSB site was analyzed in the resulting plants. Of 620 transgenic plants, 16 plants integrated T-DNA into DSB at their I-SceI sites; because DSB induces DNA repair, these results suggest that the invading T-DNA molecules target to the DNA repair sites for integration. Furthermore, of these 16 plants, seven plants incorporated T-DNA digested with I-SceI, which cleaves only double-stranded DNA. Thus, T-strand molecules can be converted into double-stranded intermediates before their integration into the DSB sites within the host cell genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.