ArticleNucleic acids research1992
Structure and function of the murine beta-globin locus control region 5' HS-3.
Article in Nucleic acids research, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 58 citations in OpenAlex.
- Systematic transfer of prokaryotic sensors and circuits to mammalian cells.ACS synthetic biology · 2014Article
- Function-based identification of mammalian enhancers using site-specific integration.Nature methods · 2014Article
- Hemoglobin induction in mouse macrophages.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Deregulation of the proto-oncogene c-myc through t(8;22) translocation in Burkitt's lymphoma.Oncogene · 1999Article
- Mechanisms of developmental control of transcription in the murine alpha- and beta-globin loci.Genes & development · 1999Article
- The locus control region is necessary for gene expression in the human beta-globin locus but not the maintenance of an open chromatin structure in erythroid cells.Molecular and cellular biology · 1998Article
- Multiple regions of p45 NF-E2 are required for beta-globin gene expression in erythroid cells.Nucleic acids research · 1997Article
- Synergism between hypersensitive sites confers long-range gene activation by the beta-globin locus control region.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Sequences within and flanking hypersensitive sites 3 and 2 of the beta-globin locus control region required for synergistic versus additive interaction with the epsilon-globin gene promoter.Nucleic acids research · 1996Article
- Analysis of mice containing a targeted deletion of beta-globin locus control region 5' hypersensitive site 3.Molecular and cellular biology · 1996Article
- Dependence of globin gene expression in mouse erythroleukemia cells on the NF-E2 heterodimer.Molecular and cellular biology · 1995Article
- The erythroid Krüppel-like factor transactivation domain is a critical component for cell-specific inducibility of a beta-globin promoter.Molecular and cellular biology · 1995Article
- NF-E2 and GATA binding motifs are required for the formation of DNase I hypersensitive site 4 of the human beta-globin locus control region.The EMBO journal · 1995Article
- Dual promoter activation by the human beta-globin locus control region.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Use of yeast artificial chromosomes (YACs) for studying control of gene expression: correct regulation of the genes of a human beta-globin locus YAC following transfer to mouse erythroleukemia cell lines.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Comparative analysis of the locus control region of the rabbit beta-like gene cluster: HS3 increases transient expression of an embryonic epsilon-globin gene.Nucleic acids research · 1993Article
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3 authors at 1 institution in 1 country.
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Abstract
We previously identified the murine homologue of the human beta-globin Locus Control Region (LCR) 5' HS-2. The lambda clone containing murine 5' HS-2 extends approximately 12 kb upstream from this site; here, we report the sequence of this entire upstream region. The murine homologue of 5' HS-3 is located approximately 16.0 kb upstream from the mouse epsilon y-globin gene, but no region homologous to human 5' HS-4 was present in our clone. Using a reporter system consisting of a human gamma-globin promoter driving the neomycin phosphotransferase gene (gamma-neo), we tested murine LCR fragments extending from -21 to -9 kb (with respect to the epsilon y-globin gene cap site) for activity in classical enhancer and integration site assays in K562 and MEL cells. 5' HS-2 behaved as a powerful enhancer and increased the number of productive integration events (as measured by a colony assay) in both K562 and MEL cells. 5' HS-3 had no activity in K562 cells or in transiently transfected MEL cells, but was nearly as active as 5' HS-2 in the MEL cell colony assay. Two additional tests confirmed the identification of murine 5' HS-3: first, a DNA fragment containing 5' HS-3 confers copy number-dependent, integration-site independent inducibility on a linked beta-globin gene in the MEL cell environment. Secondly, a strong DNAseI hypersensitive site maps to the location of the 5' HS-3 functional core in chromatin derived from MEL cells. Collectively, these data suggest that we have identified the murine homologue of human 5' HS-3, and that this site is functional when integrated into the chromatin of MEL cells but not K562 cells. 5' HS-3 may therefore contain information that contributes to the development-specific expression of the beta-like globin genes.
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