ArticleThe Biochemical journal2004
A membrane proximal region of the integrin alpha5 subunit is important for its interaction with nischarin.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Modulating PAK1: Accessory Proteins as Promising Therapeutic Targets.Biomolecules · 2025Review
- Impact of Nischarin on EMT regulators in breast cancer cell lines.Oncology letters · 2020Article
- Nischarin attenuates apoptosis induced by oxidative stress in PC12 cells.Experimental and therapeutic medicine · 2019Article
- Nischarin regulates focal adhesion and Invadopodia formation in breast cancer cells.Molecular cancer · 2018Article
- Nischarin inhibition alters energy metabolism by activating AMP-activated protein kinase.The Journal of biological chemistry · 2017Article
- Expression of integrin-binding protein Nischarin in metastatic breast cancer.Molecular medicine reports · 2015Article
- Effect of ITGA5 down-regulation on the migration capacity of human dental pulp stem cells.International journal of clinical and experimental pathology · 2015Article
- Inhibition of Nischarin Expression Promotes Neurite Outgrowth through Regulation of PAK Activity.PloS one · 2015Article
- Identification of novel integrin binding partners for calcium and integrin binding protein 1 (CIB1): structural and thermodynamic basis of CIB1 promiscuity.Biochemistry · 2013Article
- Rac and Rab GTPases dual effector Nischarin regulates vesicle maturation to facilitate survival of intracellular bacteria.The EMBO journal · 2013Article
- Structural basis for endosomal trafficking of diverse transmembrane cargos by PX-FERM proteins.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Integrin α5β1, the Fibronectin Receptor, as a Pertinent Therapeutic Target in Solid Tumors.Cancers · 2013Article
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- Molecular characterization of the tumor-suppressive function of nischarin in breast cancer.Journal of the National Cancer Institute · 2011Article
- Promoter DNA methylation of oncostatin m receptor-beta as a novel diagnostic and therapeutic marker in colon cancer.PloS one · 2009Article
- Genome-wide promoter analysis uncovers portions of the cancer methylome.Cancer research · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In a previous study [Alahari, Lee and Juliano (2000) J. Cell Biol. 151, 1141-1154], we have identified a novel protein, nischarin, that specifically interacts with the cytoplasmic tail of the alpha5 integrin subunit. Overexpression of this protein profoundly affects cell migration. To examine the nischarin-alpha5 interaction in detail, and to find the minimal region required for the interaction, several mutants of nischarin and alpha5 were created. The results obtained for the yeast two-hybrid system indicate that a 99-aminoacid region of nischarin (from residues 464 to 562) is indispensable for the interaction. Also, we demonstrate that the membrane proximal region (from residues 1017 to 1030) of the alpha5 cytoplasmic tail is essential for the interaction. To characterize more directly the properties of the interaction between nischarin and alpha5, we performed surface-plasmon resonance studies in which peptides were immobilized on the surface of a sensor chip, and the recombinant nischarin protein fragments were injected. Consistent with the two-hybrid results, recombinant nischarin binds well to immobilized alpha5 peptides. In addition, mutational analysis revealed that residues Tyr(1018) and Lys(1022) are crucial for alpha5-nischarin interactions. These results provide evidence that nischarin is capable of directly and selectively binding to a portion of the alpha5 cytoplasmic domain. Further studies demonstrated that the minimal alpha5 binding region of nischarin does not affect cell migration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.