ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2003
Fast synaptic currents in Drosophila mushroom body Kenyon cells are mediated by alpha-bungarotoxin-sensitive nicotinic acetylcholine receptors and picrotoxin-sensitive GABA receptors.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 114 citations in OpenAlex.
- A highly sensitive genetically encoded red cAMP sensor for multiplex imaging in vivo.Nature communications · 2026Article
- The cellular architecture of memory modules ineLife · 2023Article
- Minimal circuit motifs for second-order conditioning in the insect mushroom body.Frontiers in physiology · 2023Article
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- Effects of Mono-2-ethylhexyl Phthalate on the Neural Transmission of PNs in Drosophila Antennal Lobe.Neurotoxicity research · 2021Article
- Constructing and Tuning Excitatory Cholinergic Synapses: The Multifaceted Functions of Nicotinic Acetylcholine Receptors inFrontiers in cellular neuroscience · 2021Review
- Carbon Monoxide, a Retrograde Messenger Generated in Postsynaptic Mushroom Body Neurons, Evokes Noncanonical Dopamine Release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- The Neurotransmitters Involved inFrontiers in behavioral neuroscience · 2020Review
- Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adulteLife · 2019Article
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- Synaptic depression induced by postsynaptic cAMP production in the Drosophila mushroom body calyx.The Journal of physiology · 2018Article
- Electrophysiological analysis of synaptic transmission in Drosophila.Wiley interdisciplinary reviews. Developmental biology · 2017Review
- Coincident postsynaptic activity gates presynaptic dopamine release to induce plasticity ineLife · 2017Article
- Developmental experience-dependent plasticity in the first synapse of the Drosophila olfactory circuit.Journal of neurophysiology · 2016Review
- Long-term memory formation in Drosophila requires training-dependent glial transcription.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Muscarinic ACh Receptors Contribute to Aversive Olfactory Learning in Drosophila.Neural plasticity · 2015Article
- Honeybee Kenyon cells are regulated by a tonic GABA receptor conductance.Journal of neurophysiology · 2014Article
- Larval Helicoverpa zea Transcriptional, Growth and Behavioral Responses to Nicotine and Nicotiana tabacum.Insects · 2014Article
- A biophysical analysis of mitochondrial movement: differences between transport in neuronal cell bodies versus processes.Traffic (Copenhagen, Denmark) · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The mushroom bodies, bilaterally symmetric regions in the insect brain, play a critical role in olfactory associative learning. Genetic studies in Drosophila suggest that plasticity underlying acquisition and storage of memory occurs at synapses on the dendrites of mushroom body Kenyon cells (Dubnau et al., 2001). Additional exploration of the mechanisms governing synaptic plasticity contributing to these aspects of olfactory associative learning requires identification of the receptors that mediate fast synaptic transmission in Kenyon cells. To this end, we developed a culture system that supports the formation of excitatory and inhibitory synaptic connections between neurons harvested from the central brain region of late-stage Drosophila pupae. Mushroom body Kenyon cells are identified as small-diameter, green fluorescent protein-positive (GFP+) neurons in cultures from OK107-GAL4;UAS-GFP pupae. In GFP+ Kenyon cells, fast EPSCs are mediated by alpha-bungarotoxin-sensitive nicotinic acetylcholine receptors (nAChRs). The miniature EPSCs have rapid rise and decay kinetics and a broad, positively skewed amplitude distribution. Fast IPSCs are mediated by picrotoxin-sensitive chloride conducting GABA receptors. The miniature IPSCs also have a rapid rate of rise and decay and a broad amplitude distribution. The vast majority of spontaneous synaptic currents in the cultured Kenyon cells are mediated byalpha-bungarotoxin-sensitive nAChRs or picrotoxin-sensitive GABA receptors. Therefore, these receptors are also likely to mediate synaptic transmission in Kenyon cells in vivo and to contribute to plasticity during olfactory associative learning.
Indexed as
Identifiers
14534259PMC6740836W1943715850What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.