ArticleJournal of clinical microbiology2003
Detection of Yersinia pestis in sputum by real-time PCR.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 83 citations in OpenAlex.
- Surveillance and Analysis of Plague Epidemic - China, 2010-2024.China CDC weekly · 2026Article
- Development and evaluation of a triplex real-time PCR assay for enhanced plague diagnostics in Madagascar.PLoS neglected tropical diseases · 2025Article
- Climatic variations and Yersinia pestis host-vector abundance: a case study in Ankazobe district to understand plague epidemiology in Madagascar.BMC infectious diseases · 2025Article
- Multiplex PCR System for the Diagnosis of Plague.Current molecular medicine · 2025Review
- Analytical framework to evaluate and optimize the use of imperfect diagnostics to inform outbreak response: Application to the 2017 plague epidemic in Madagascar.PLoS biology · 2022Article
- Applications of polymerase chain reaction-based methods for the diagnosis of plague (Review).Experimental and therapeutic medicine · 2022Review
- Obtaining Specific Sequence Tags forPathogens (Basel, Switzerland) · 2021Article
- Yersinia pestis: the Natural History of Plague.Clinical microbiology reviews · 2020Review
- Review
- Human plague: An old scourge that needs new answers.PLoS neglected tropical diseases · 2020Review
- Epidemiological characteristics of an urban plague epidemic in Madagascar, August-November, 2017: an outbreak report.The Lancet. Infectious diseases · 2019Article
- Yersinia pestis and plague: an updated view on evolution, virulence determinants, immune subversion, vaccination, and diagnostics.Genes and immunity · 2019Review
- Rapid and sensitive detection of Yersinia pestis by lateral-flow assay in simulated clinical samples.BMC infectious diseases · 2018Article
- Plague in China 2014-All sporadic case report of pneumonic plague.BMC infectious diseases · 2016Article
- Article
- Genomic Insights into a New Citrobacter koseri Strain Revealed Gene Exchanges with the Virulence-Associated Yersinia pestis pPCP1 Plasmid.Frontiers in microbiology · 2016Article
- The pla gene, encoding plasminogen activator, is not specific to Yersinia pestis.BMC research notes · 2015Article
- Preliminary Survey of Ectoparasites and Associated Pathogens from Norway Rats in New York City.Journal of medical entomology · 2015Article
- Yersinia pestis plasminogen activator gene homolog in rat tissues.Emerging infectious diseases · 2013Article
- Phage-based platforms for the clinical detection of human bacterial pathogens.Bacteriophage · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 5' nuclease PCR assay for detection of the Yersinia pestis plasminogen activator (pla) gene in human respiratory specimens with simulated Y. pestis infection was developed. An internal positive control was added to the reaction mixture in order to detect the presence of PCR inhibitors that are often found in biological samples. The assay was 100% specific for Y. pestis. In the absence of inhibitors, a sensitivity of 10(2) CFU/ml of respiratory fluid was obtained. When inhibitors were present, detection of Y. pestis DNA required a longer sample treatment time and an initial concentration of bacteria of at least 10(4) CFU/ml. The test's total turnaround time was less than 5 h. The assay described here is well suited to the rapid diagnosis of pneumonic plague, the form of plague most likely to result from a bioterrorist attack.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.