ArticleJournal of clinical microbiology2003
Optimization of real-time PCR assay for rapid and sensitive detection of eubacterial 16S ribosomal DNA in platelet concentrates.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed.
- Microbial Ecosystem Therapeutics 4 (MET4) elicits treatment-specific IgG responses associated with changes in gut microbiota in immune checkpoint inhibitor recipients with advanced solid tumors.Journal for immunotherapy of cancer · 2025Article
- Bacterial viability in the built environment of the home.PloS one · 2023Article
- Article
- Characterization of the Ovine Vaginal Microbiome and Inflammation Patterns as an Improved Testing Model of Human Vaginal Irritation.Frontiers in reproductive health · 2021Article
- Clinical metagenomics for infectious corneal ulcers: Rags to riches?The ocular surface · 2020Article
- Multimodal Magneto-Fluorescent Nanosensor for Rapid and Specific Detection of Blood-Borne Pathogens.ACS applied nano materials · 2019Article
- Establishment of a method for measuring leukocyte phagocytosis based on 16S rDNA.Molecular biology reports · 2019Article
- Massively parallel digital high resolution melt for rapid and absolutely quantitative sequence profiling.Scientific reports · 2017Article
- Enhancement of PCR Detection Limit by Single-Tube Restriction Endonuclease-PCR (RE-PCR).Molecular diagnosis & therapy · 2016Article
- Automated Broad-Range Molecular Detection of Bacteria in Clinical Samples.Journal of clinical microbiology · 2016Article
- Usefulness of Multiplex Real-Time PCR for Simultaneous Pathogen Detection and Resistance Profiling of Staphylococcal Bacteremia.BioMed research international · 2016Article
- An Internal Reference Control Duplex Real-Time Polymerase Chain Reaction Assay for Detecting Bacterial Contamination in Blood Products.PloS one · 2015Article
- Article
- Reagent and laboratory contamination can critically impact sequence-based microbiome analyses.BMC biology · 2014Article
- Multiplex real-time PCR assay for rapid detection of methicillin-resistant staphylococci directly from positive blood cultures.Journal of clinical microbiology · 2014Article
- High-fat maternal diet during pregnancy persistently alters the offspring microbiome in a primate model.Nature communications · 2014Article
- Article
- Real-time PCR TaqMan assay for rapid screening of bloodstream infection.Annals of clinical microbiology and antimicrobials · 2014Article
- Evaluation of a broad range real-time polymerase chain reaction (RT-PCR) assay for the diagnosis of septic synovitis in horses.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2013Article
- The effect of rifaximin on gut flora and Staphylococcus resistance.Digestive diseases and sciences · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A real-time PCR assay was developed for rapid detection of eubacterial 16S ribosomal DNA in platelet concentrates. The sensitivity of this assay can be hampered by contaminating DNA in the PCR reagents. Digestion of the PCR reagents with Sau3AI prior to PCR amplification was effective in eliminating this contaminating DNA without affecting the sensitivity of the assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.