ArticleJournal of clinical microbiology2003
Rapid and specific detection of Mycobacterium tuberculosis by using the Smart Cycler instrument and a specific fluorogenic probe.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Diagnostic accuracy of in-house PCR for pulmonary tuberculosis in smear-positive patients: meta-analysis and metaregression.Journal of clinical microbiology · 2009Pooled it
- Development of a TaqMan Array card to target 21 purulent meningitis-related pathogens.BMC infectious diseases · 2019Article
- Emergence of Orientia tsutsugamushi as an important cause of Acute Encephalitis Syndrome in India.PLoS neglected tropical diseases · 2018Article
- Comparison of two molecular methods for rapid diagnosis of extrapulmonary tuberculosis.Journal of clinical microbiology · 2011Article
- Evaluation of the rapid MGIT TBc identification test for culture confirmation of Mycobacterium tuberculosis complex strain detection.Journal of clinical microbiology · 2011Article
- A novel multiplex real-time PCR for the identification of mycobacteria associated with zoonotic tuberculosis.PloS one · 2011Article
- Combined real-time PCR and rpoB gene pyrosequencing for rapid identification of Mycobacterium tuberculosis and determination of rifampin resistance directly in clinical specimens.Journal of clinical microbiology · 2010Article
- Evaluation of multiplex real-time PCR for detection of Haemophilus ducreyi, Treponema pallidum, herpes simplex virus type 1 and 2 in the diagnosis of genital ulcer disease in the Rakai District, Uganda.Sexually transmitted infections · 2009Article
- Multi-probe real-time PCR identification of common Mycobacterium species in blood culture broth.The Journal of molecular diagnostics : JMD · 2009Article
- Molecular-beacon-based real-time PCR for detection and quantification of Mycobacterium tuberculosis DNA in clinical samples.Journal of clinical microbiology · 2008Article
- Review
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Comparison of six methods of extracting Mycobacterium tuberculosis DNA from processed sputum for testing by quantitative real-time PCR.Journal of clinical microbiology · 2005Article
- Comparison of an internally controlled, large-volume LightCycler assay for detection of Mycobacterium tuberculosis in clinical samples with the COBAS AMPLICOR assay.Journal of clinical microbiology · 2005Article
- Comparison of the real-time PCR method and the Gen-Probe amplified Mycobacterium tuberculosis direct test for detection of Mycobacterium tuberculosis in pulmonary and nonpulmonary specimens.Journal of clinical microbiology · 2004Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A procedure using the Smart Cycler instrument and a fluorescence quencher (FQ) probe for the specific identification of Mycobacterium tuberculosis complex (MTB) was used to detect organisms in 366 acid-fast bacillus smear-positive respiratory specimens. It was compared to culture and the AMPLICOR M. tuberculosis PCR test. MTB was isolated from 198 of these samples. The FQ PCR assay was sensitive (197 of 198, 99.5%) and specific (165 of 168, 98.2%); no significant difference was observed between the two PCR protocols. After DNA extraction, a final result was available within 1.5 h with the real-time PCR protocol.
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