Evidence map›Paper›PMID 14532217›Full record

ArticleJournal of clinical microbiology2003

Use of denaturing high-performance liquid chromatography to identify Bacillus anthracis by analysis of the 16S-23S rRNA interspacer region and gyrA gene.

William Hurtle, Elizabeth Bode, Rebecca Susan Kaplan, Jeff Garrison, Brian Kearney, David Shoemaker, Erik Henchal, David Norwood

Abstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

William HurtleClinical Research Management, North Royalton, Ohio 44133, USA.
Elizabeth Bode
Rebecca Susan Kaplan
Jeff Garrison
Brian Kearney
David Shoemaker
Erik Henchal
David Norwood

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Denaturing high-performance liquid chromatography (DHPLC) was evaluated as a method for identifying Bacillus anthracis by analyzing two chromosomal targets, the 16S-23S intergenic spacer region (ISR) and the gyrA gene. The 16S-23S ISR was analyzed by this method with 42 strains of B. anthracis, 36 strains of Bacillus cereus, and 12 strains of Bacillus thuringiensis; the gyrA gene was analyzed by this method with 33 strains of B. anthracis, 27 strains of B. cereus, and 9 strains of B. thuringiensis. Two blind panels of 45 samples each were analyzed to evaluate the potential diagnostic capability of this method. Our results show that DHPLC is an efficient method for the identification of B. anthracis.

Indexed as

Bacterial Typing TechniquesBacillus anthracisChromatography, High Pressure LiquidDNA GyraseDNA, Ribosomal SpacerMolecular Sequence DataRNA, Ribosomal, 16SRNA, Ribosomal, 23SSequence Analysis, DNADNA GyraseDNA, Ribosomal SpacerRNA, Ribosomal, 16SRNA, Ribosomal, 23S

Identifiers

PMID14532217
PMCPMC254356

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.