ArticleJournal of clinical microbiology2003
Utility of multilocus sequence typing as an epidemiological tool for investigation of outbreaks of gastroenteritis caused by Campylobacter jejuni.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 1 of them a synthesis that pooled it.
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Who cites it
52 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Large-scale comparative genomics meta-analysis of Campylobacter jejuni isolates reveals low level of genome plasticity.Journal of clinical microbiology · 2004Pooled it
- Aerotolerance and Multi-Locus Sequence Typing ofFoods (Basel, Switzerland) · 2023Article
- A Systematic Review on the Role of Wildlife as Carriers and Spreaders ofAnimals : an open access journal from MDPI · 2023Review
- Molecular subtyping for source tracking of Escherichia coli using core genome multilocus sequence typing at a food manufacturing plant.PloS one · 2021Article
- Molecular typing of Campylobacter jejuni strains: comparison among four different techniques.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Evaluation of WGS-subtyping methods for epidemiological surveillance of foodborne salmonellosis.One health outlook · 2020Article
- Comparison of different technologies for the decipherment of the whole genome sequence ofGut pathogens · 2019Article
- The EpiQuant Framework for Computing Epidemiological Concordance of Microbial Subtyping Data.Journal of clinical microbiology · 2017Article
- Complete Annotated Genome Sequences of Three Campylobacter jejuni Strains Isolated from Naturally Colonized Farm-Raised Chickens.Genome announcements · 2017Article
- Molecular Subtyping and Source Attribution of Campylobacter Isolated from Food Animals.Journal of food protection · 2016Article
- Comparison of epidemiologically linked Campylobacter jejuni isolated from human and poultry sources.Epidemiology and infection · 2015Article
- Partial Failure of Milk Pasteurization as a Risk for the Transmission of Campylobacter From Cattle to Humans.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2015Article
- High Prevalence of Hyper-Aerotolerant Campylobacter jejuni in Retail Poultry with Potential Implication in Human Infection.Frontiers in microbiology · 2015Article
- Comprehensive detection and discrimination of Campylobacter species by use of confocal micro-Raman spectroscopy and multilocus sequence typing.Journal of clinical microbiology · 2012Article
- Transmission OF Campylobacter coli in chicken embryos.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2012Article
- Comparison of molecular typing methods useful for detecting clusters of Campylobacter jejuni and C. coli isolates through routine surveillance.Journal of clinical microbiology · 2012Article
- Development and validation of a comparative genomic fingerprinting method for high-resolution genotyping of Campylobacter jejuni.Journal of clinical microbiology · 2012Article
- Article
- A framework for assessing the concordance of molecular typing methods and the true strain phylogeny of Campylobacter jejuni and C. coli using draft genome sequence data.Frontiers in cellular and infection microbiology · 2012Article
- High-resolution melting system to perform multilocus sequence typing of Campylobacter jejuni.PloS one · 2011Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multilocus sequence typing (MLST) has been proven useful for the study of the global population structure of Campylobacter jejuni; however, its usefulness for the investigation of outbreaks of disease caused by C. jejuni has not been proven. In this study, MLST plus sequencing of the flaA short variable region (SVR) were applied to 47 isolates from 12 outbreaks of C. jejuni infection whose relatedness has been determined previously, and the results were compared to those of serotyping and pulsed-field gel electrophoresis (PFGE). Isolates implicated in an outbreak were indistinguishable by all four subtyping methods, with sporadic isolates being distinguished from outbreak isolates. Two sporadic isolates from one outbreak were resistant to SmaI digestion and therefore nontypeable by PFGE but were differentiated from the outbreak strain by the other methods. PFGE and flaA SVR typing were the most discriminatory methods, with discriminatory indices (DI) of 0.930 and 0.923, respectively. However, an epidemic strain from one outbreak was distinguished from the other outbreak isolates by flaA SVR typing; its flaA allele was different at five nucleotides, suggesting that this change was possibly mediated by recombination. MLST was less discriminatory than PFGE and flaA SVR typing (DI = 0.859), and many of the epidemic strains possessed common sequence types (STs) including ST-8, -21, -22, and -42. However, further discrimination within STs was achieved by flaA SVR typing or PFGE. The results from this study demonstrate that a combined approach of MLST plus flaA SVR typing provides a level of discrimination equivalent to PFGE for outbreak investigations.
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