ArticleJournal of clinical microbiology2003
Rapid diagnosis of mycobacterial infections and quantitation of Mycobacterium tuberculosis load by two real-time calibrated PCR assays.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 89 citations in OpenAlex.
- Towards culture-free sequencing of Mycobacterium tuberculosis: evaluating new targeted and whole-genome approaches for genotyping and drug resistance profiling.Genome medicine · 2026Article
- From Tradition to Innovation: Diverse Molecular Techniques in the Fight Against Infectious Diseases.Diagnostics (Basel, Switzerland) · 2024Review
- Evaluation of Nanopore Sequencing as a Diagnostic Tool for the Rapid Identification of Mycoplasma bovis from Individual and Pooled Respiratory Tract Samples.Journal of clinical microbiology · 2021Article
- Revisiting the methods for detectingAccess microbiology · 2021Review
- Diversity of Mycobacterium tuberculosis in the Middle Fly District of Western Province, Papua New Guinea: microbead-based spoligotyping using DNA from Ziehl-Neelsen-stained microscopy preparations.Scientific reports · 2019Article
- Molecular Evidence of Drug-Resistant Tuberculosis in the Balimo Region of Papua New Guinea.Tropical medicine and infectious disease · 2019Article
- Quantifying intracellular Mycobacterium tuberculosis: An essential issue for in vitro assays.MicrobiologyOpen · 2018Review
- Optimized Lysis-Extraction Method Combined WithFrontiers in microbiology · 2018Article
- Use of Digital Droplet PCR to DetectFrontiers in cellular and infection microbiology · 2017Article
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- Molecular diagnosis of central nervous system opportunistic infections in HIV-infected Zambian adults.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014Article
- Potential for use of the Seegene Anyplex MTB/NTM real-time detection assay in a regional reference laboratory.Journal of clinical microbiology · 2014Article
- Mycobacterium tuberculosis infection is associated with the development of erythema nodosum and nodular vasculitis.PloS one · 2013Article
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- Assessment of Status of rpoB Gene in FNAC Samples of Tuberculous Lymphadenitis by Real-Time PCR.Tuberculosis research and treatment · 2012Article
- The role of TLR4 activation in photoreceptor mitochondrial oxidative stress.Investigative ophthalmology & visual science · 2011Article
- Rapid, sensitive, and specific detection of Mycobacterium tuberculosis complex by real-time PCR on paraffin-embedded human tissues.The Journal of molecular diagnostics : JMD · 2011Article
- Detection of bacillus Galmette-Guérin (Mycobacterium bovis BCG) DNA in urine and blood specimens after intravesical immunotherapy for bladder carcinoma.Journal of clinical microbiology · 2011Article
- An appraisal of PCR-based technology in the detection of Mycobacterium tuberculosis.Molecular diagnosis & therapy · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sensitive and specific techniques to detect and identify Mycobacterium tuberculosis directly in clinical specimens are important for the diagnosis and management of patients with tuberculosis (TB). We developed two real-time PCR assays, based on the IS6110 multicopy element and on the senX3-regX3 intergenic region, which provide a rapid method for the diagnosis of mycobacterial infections. The sensitivity and specificity of both assays were established by using purified DNA from 71 clinical isolates and 121 clinical samples collected from 83 patients, 20 of whom were affected by TB. Both assays are accurate, sensitive, and specific, showing a complementary pattern of Mycobacterium recognition: broader for the IS6110-based assay and restricted to the M. tuberculosis complex for the senX3-regX3-based assay. Moreover, the addition of a synthetic DNA calibrator prior to DNA extraction allowed us to measure the efficiency of DNA recovery and to control for the presence of PCR inhibitors. The mycobacterial burden of the clinical samples, as assessed by direct microscopy, correlates with the M. tuberculosis DNA load measured by the senX3-regX3-based assay. In addition, reduced levels of M. tuberculosis DNA load are present in those patients subjected to successful therapy, suggesting a potential use of this assay for monitoring treatment efficacy. Therefore, these assays represent a fully controlled high-throughput system for the evaluation of mycobacterial burden in clinical specimens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.