ArticleJournal of clinical microbiology2003
Molecular detection and identification of influenza viruses by oligonucleotide microarray hybridization.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 137 citations in OpenAlex.
- Review
- Using NMR and molecular dynamics to link structure and dynamics effects of the universal base 8-aza, 7-deaza, N8 linked adenosine analog.Nucleic acids research · 2016Article
- DNA microarray for detection of gastrointestinal viruses.Journal of clinical microbiology · 2015Article
- Development and clinical testing of a simple, low-density gel element array for influenza identification, subtyping, and H275Y detection.Journal of virological methods · 2014Article
- PhyloFlu, a DNA microarray for determining the phylogenetic origin of influenza A virus gene segments and the genomic fingerprint of viral strains.Journal of clinical microbiology · 2014Article
- Comparative evaluation of effectiveness of IAVchip DNA microarray in influenza A diagnosis.TheScientificWorldJournal · 2014Article
- Evolving gene targets and technology in influenza detection.Molecular diagnosis & therapy · 2013Review
- Design of a set of probes with high potential for influenza virus epidemiological surveillance.Bioinformation · 2013Article
- Nucleic acid sandwich hybridization assay with quantum dot-induced fluorescence resonance energy transfer for pathogen detection.Sensors (Basel, Switzerland) · 2012Article
- Development of a flow-through [corrected] microarray based reverse transcriptase multiplex ligation-dependent probe amplification assay for the detection of European Bunyaviruses. [corrected].Molecular biotechnology · 2011Article
- Improvement of the specificity of a pan-viral microarray by using genus-specific oligonucleotides and reduction of interference by host genomes.Journal of medical virology · 2011Article
- Article
- Facile DNA immobilization on surfaces through a catecholamine polymer.Angewandte Chemie (International ed. in English) · 2011Article
- A microbial detection array (MDA) for viral and bacterial detection.BMC genomics · 2010Article
- Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay.BMC biotechnology · 2010Article
- Future Veterinary Diagnostics.Journal of exotic pet medicine · 2010Article
- Oligonucleotide-based microarray for detection of plant viruses employing sequence-independent amplification of targets.Journal of virological methods · 2010Article
- Development of a consensus microarray method for identification of some highly pathogenic viruses.Journal of medical virology · 2009Article
- Basic concepts of microarrays and potential applications in clinical microbiology.Clinical microbiology reviews · 2009Review
- Universal detection and identification of avian influenza virus by use of resequencing microarrays.Journal of clinical microbiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microarrays of virus-specific oligonucleotides may provide a method of screening samples for the presence or absence of a large variety of viruses simultaneously. Influenza viruses are ideal for evaluating such microarrays because of their genetic and host diversity, and the availability of an extensive sequence database. A collection of 476 influenza virus-specific oligonucleotides was spotted onto glass slides as probes. Viral RNAs were reverse transcribed and amplified by PCR, and the products were labeled with cyanine dyes. The presence of viruses and their identities were determined by hybridization. The fluorescence intensities of oligonucleotide spots were highly reproducible within each slide and satisfactorily proportional between experiments. However, the intensities of probe spots completely complementary to target sequences varied from background to saturation. The variations did not correlate with base composition, nucleotide sequence, or internal secondary structures. Therefore, thresholds for determining whether hybridization to a spot should be judged as positive were assigned individually. Considering only positive spots from probes predicted to be monospecific for influenza virus species, subtype, host source, or gene segment, this method made correct identifications at the species, hemagglutinin subtype, and gene segment levels. Monospecific neuraminidase (NA) subtype probes were insufficiently diverse to allow confident NA subtype assignment. Incorporating positive spots from polyspecific probes into the identification scheme gave similar results. Overall, the results demonstrate the potential of microarray-based oligonucleotide hybridization for multiple virus detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.