ArticleThe EMBO journal2003
A replication silencer element in a plus-strand RNA virus.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 130 citations in OpenAlex.
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- Structural Analysis and Whole Genome Mapping of a New Type of Plant Virus Subviral RNA: Umbravirus-Like Associated RNAs.Viruses · 2021Article
- The Pseudo-Circular Genomes of Flaviviruses: Structures, Mechanisms, and Functions of Circularization.Cells · 2021Review
- Complete Nucleotide Sequence, Genome Organization, and Comparative Genomic Analyses of Citrus Yellow-Vein Associated Virus (CYVaV).Frontiers in microbiology · 2021Article
- Identification of therapeutics that target eEF1A2 and upregulate utrophin A translation in dystrophic muscles.Nature communications · 2020Article
- Chimeric Tobamoviruses With Coat Protein Exchanges Modulate Symptom Expression and Defence Responses inFrontiers in microbiology · 2020Article
- Host protein chaperones, RNA helicases and the ubiquitin network highlight the arms race for resources between tombusviruses and their hosts.Advances in virus research · 2020Article
- Variable 3'polyadenylation of Wheat yellow mosaic virus and its novel effects on translation and replication.Virology journal · 2019Article
- NF90-NF45 is a selective RNA chaperone that rearranges viral and cellular riboswitches: biochemical analysis of a virus host factor activity.Nucleic acids research · 2017Article
- Atypical RNA Elements Modulate Translational Readthrough in Tobacco Necrosis Virus D.Journal of virology · 2017Article
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- Biologically-supported structural model for a viral satellite RNA.Nucleic acids research · 2015Article
- Binding of the eukaryotic translation elongation factor 1A with the 5'UTR of HIV-1 genomic RNA is important for reverse transcription.Virology journal · 2015Article
- Host and viral RNA-binding proteins involved in membrane targeting, replication and intercellular movement of plant RNA virus genomes.Frontiers in plant science · 2014Review
- The unexpected roles of eukaryotic translation elongation factors in RNA virus replication and pathogenesis.Microbiology and molecular biology reviews : MMBR · 2013Review
- p33-Independent activation of a truncated p92 RNA-dependent RNA polymerase of Tomato bushy stunt virus in yeast cell-free extract.Journal of virology · 2012Article
- A Co-Opted DEAD-Box RNA helicase enhances tombusvirus plus-strand synthesis.PLoS pathogens · 2012Article
- Defining the roles of cis-acting RNA elements in tombusvirus replicase assembly in vitro.Journal of virology · 2012Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Replication represents a key step in the infectious cycles of RNA viruses. Here we describe a regulatory RNA element, termed replication silencer, that can down-regulate complementary RNA synthesis of a positive-strand RNA virus via an RNA-RNA interaction. This interaction occurs between the 5-nucleotide-long, internally positioned replication silencer and the extreme 3'-terminus of the viral RNA comprising part of the minimal minus-strand initiation promoter. Analysis of RNA synthesis in vitro, using model defective interfering (DI) RNA templates of tomato bushy stunt virus and a partially purified, RNA-dependent RNA polymerase preparation from tombusvirus-infected plants, revealed that this interaction inhibits minus-strand synthesis 7-fold. This functional interaction was supported further by: (i) RNA structure probing; (ii) phylogenetic analysis; (iii) inhibition of activity by short complementary DNAs; and (iv) compensatory mutational analysis. The silencer was found to be essential for accumulation of DI RNAs in protoplasts, indicating that it serves an important regulatory role(s) in vivo. Because similar silencer-promoter interactions are also predicted in other virus genera, this type of RNA-based regulatory mechanism may represent a widely utilized strategy for modulating replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.