ArticleThe EMBO journal2003
Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Mechanical states of a motor protein in the spindle.Current biology : CB · 2026Article
- Structural analysis of a motor with increased mechanical output reveals new transitions in kinesin microtubule motility.Scientific reports · 2026Article
- Kinesin proteins HUG1 and HUG2 are essential for the formation and transportation of male germ units in Arabidopsis pollen tubes.Nature plants · 2025Article
- Kinesin-14 HSET and KlpA are non-processive microtubule motors with load-dependent power strokes.Nature communications · 2024Article
- Functional characterization of MCAK/Kif2C cancer mutations using high-throughput microscopic analysis.Molecular biology of the cell · 2020Article
- An estimate to the first approximation of microtubule rupture force.European biophysics journal : EBJ · 2019Article
- Structural basis of small molecule ATPase inhibition of a human mitotic kinesin motor protein.Scientific reports · 2017Article
- The mitotic kinesin-14 KlpA contains a context-dependent directionality switch.Nature communications · 2017Article
- Working stroke of the kinesin-14, ncd, comprises two substeps of different direction.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Motile properties of the bi-directional kinesin-5 Cin8 are affected by phosphorylation in its motor domain.Scientific reports · 2016Article
- Kinesin Motor Enzymology: Chemistry, Structure, and Physics of Nanoscale Molecular Machines.Biophysical reviews · 2015Article
- Drosophila Ncd reveals an evolutionarily conserved powerstroke mechanism for homodimeric and heterodimeric kinesin-14s.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Remote control of myosin and kinesin motors using light-activated gearshifting.Nature nanotechnology · 2014Article
- Kar3Vik1 mechanochemistry is inhibited by mutation or deletion of the C terminus of the Vik1 subunit.The Journal of biological chemistry · 2013Article
- Article
- Force generation by kinesin and myosin cytoskeletal motor proteins.Journal of cell science · 2013Review
- Article
- Neck rotation and neck mimic docking in the noncatalytic Kar3-associated protein Vik1.The Journal of biological chemistry · 2012Article
- The ATPase pathway that drives the kinesin-14 Kar3Vik1 powerstroke.The Journal of biological chemistry · 2012Article
- Kar3Vik1, a member of the kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern.The Journal of cell biology · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Molecular motors undergo conformational changes to produce force and move along cytoskeletal filaments. Structural changes have been detected in kinesin motors; however, further changes are expected because previous crystal structures are in the same or closely related conformations. We report here a 2.5 A crystal structure of the minus-end kinesin, Ncd, with the coiled-coil stalk/neck and one head rotated by approximately 75 degrees relative to the other head. The two heads are asymmetrically positioned with respect to the stalk and show asymmetry of nucleotide state: one head is fully occupied, but the other is unstably bound to ADP. Unlike previous structures, our new atomic model can be fit into cryoelectron microscopy density maps of the motor attached to microtubules, where it appears to resemble a one-head-bound motor with the stalk rotated towards the minus end. Interactions between neck and motor core residues, observed in the head that moves with the stalk, are disrupted in the other head, permitting rotation of the stalk/neck. The rotation could represent a force-producing stroke that directs the motor to the minus end.
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