ArticleApplied and environmental microbiology2003
Prospecting for novel biocatalysts in a soil metagenome.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers, 1 of them a synthesis that pooled it.
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Who cites it
62 citing papers in PubMed, 1 synthesis or guideline pooled it, 303 citations in OpenAlex.
- Marine microbiomes and their expanding role in biotechnological potential: a systematic review.Archives of microbiology · 2026Pooled it
- Metagenomics approaches in the discovery and development of new bioactive compound of 8-demethoxy-10-deoxysteffimycin from mangrove sediments.3 Biotech · 2024Article
- Screening Metagenomes for Algae Cell Wall Carbohydrates Degrading Hydrolases in Enrichment Cultures.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Potential Use of Microbial Community Genomes in Various Dimensions of Agriculture Productivity and Its Management: A Review.Frontiers in microbiology · 2022Review
- Thermostable lipases and their dynamics of improved enzymatic properties.Applied microbiology and biotechnology · 2021Review
- Advances in Metagenomics and Its Application in Environmental Microorganisms.Frontiers in microbiology · 2021Review
- Activity-Based Protein Profiling for the Identification of Novel Carbohydrate-Active Enzymes Involved in Xylan Degradation in the Hyperthermophilic EuryarchaeonFrontiers in microbiology · 2021Article
- Emerging priorities for microbial metagenome research.Bioresource technology reports · 2020Review
- A novel bacterial carboxylesterase identified in a metagenome derived-clone from Brazilian mangrove sediments.Molecular biology reports · 2020Article
- Thermostable endoglucanase gene derived by amplification from the genomic DNA of a cellulose-enriched mixed culture from mudspring water of Mt. Makiling, Laguna, Philippines.World journal of microbiology & biotechnology · 2020Article
- Identification and molecular characterization of a metagenome-derived L-lysine decarboxylase gene from subtropical soil microorganisms.PloS one · 2017Article
- Buwchitin: A Ruminal Peptide with Antimicrobial Potential againstFrontiers in chemistry · 2017Article
- Construction and Analysis of Functional Networks in the Gut Microbiome of Type 2 Diabetes Patients.Genomics, proteomics & bioinformatics · 2016Article
- Isolation and Characterization of a Glycosyl Hydrolase Family 16 β-Agarase from a Mangrove Soil Metagenomic Library.International journal of molecular sciences · 2016Article
- A Metagenomic Advance for the Cloning and Characterization of a Cellulase from Red Rice Crop Residues.Molecules (Basel, Switzerland) · 2016Article
- A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities.Biotechnology for biofuels · 2016Article
- A new alkaline lipase obtained from the metagenome of marine sponge Ircinia sp.World journal of microbiology & biotechnology · 2015Article
- Improved cultivation and metagenomics as new tools for bioprospecting in cold environments.Extremophiles : life under extreme conditions · 2015Review
- Metagenomics: Retrospect and Prospects in High Throughput Age.Biotechnology research international · 2015Review
- Functional Screening of Hydrolytic Activities Reveals an Extremely Thermostable Cellulase from a Deep-Sea Archaeon.Frontiers in bioengineering and biotechnology · 2015Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The metagenomes of complex microbial communities are rich sources of novel biocatalysts. We exploited the metagenome of a mixed microbial population for isolation of more than 15 different genes encoding novel biocatalysts by using a combined cultivation and direct cloning strategy. A 16S rRNA sequence analysis revealed the presence of hitherto uncultured microbes closely related to the genera Pseudomonas, Agrobacterium, Xanthomonas, Microbulbifer, and Janthinobacterium. Total genomic DNA from this bacterial community was used to construct cosmid DNA libraries, which were functionally searched for novel enzymes of biotechnological value. Our searches in combination with cosmid sequencing resulted in identification of four clones encoding 12 putative agarase genes, most of which were organized in clusters consisting of two or three genes. Interestingly, nine of these agarase genes probably originated from gene duplications. Furthermore, we identified by DNA sequencing several other biocatalyst-encoding genes, including genes encoding a putative stereoselective amidase (amiA), two cellulases (gnuB and uvs080), an alpha-amylase (amyA), a 1,4-alpha-glucan branching enzyme (amyB), and two pectate lyases (pelA and uvs119). Also, a conserved cluster of two lipase genes was identified, which was linked to genes encoding a type I secretion system. The novel gene aguB was overexpressed in Escherichia coli, and the enzyme activities were determined. Finally, we describe more than 162 kb of DNA sequence that provides a strong platform for further characterization of this microbial consortium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.