ArticleApplied and environmental microbiology2003
Use of bromodeoxyuridine immunocapture to identify active bacteria associated with arbuscular mycorrhizal hyphae.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- From dividing to dormant: embracing the full activity spectrum for environmental microorganisms.Microbiology and molecular biology reviews : MMBR · 2026Review
- Intercropping with potato-onion alters arbuscular mycorrhizal fungi spore-associated bacterial communities of tomato rhizosphere.Frontiers in microbiology · 2025Article
- Disentangling arbuscular mycorrhizal fungi and bacteria at the soil-root interface.Mycorrhiza · 2023Review
- Coculture ofMicroorganisms · 2022Article
- Years of sand fixation withPeerJ · 2022Article
- Possible role of arbuscular mycorrhizal fungi and associated bacteria in the recruitment of endophytic bacterial communities by plant roots.Mycorrhiza · 2021Review
- Targeting the Active Rhizosphere Microbiome ofFrontiers in microbiology · 2021Article
- Probing the active fraction of soil microbiomes using BONCAT-FACS.Nature communications · 2019Article
- Capturing the genetic makeup of the active microbiome in situ.The ISME journal · 2017Review
- Microbiology Meets Archaeology: Soil Microbial Communities Reveal Different Human Activities at Archaic Monte Iato (Sixth Century BC).Microbial ecology · 2017Article
- Probiotic properties of Enterococcus strains isolated from traditional naturally fermented cream in China.Microbial biotechnology · 2016Article
- Genetic Diversity and Association Characters of Bacteria Isolated from Arbuscular Mycorrhizal Fungal Spore Walls.PloS one · 2016Article
- Mycorrhizal hyphae as ecological niche for highly specialized hypersymbionts - or just soil free-riders?Frontiers in plant science · 2013Article
- Convergent development of anodic bacterial communities in microbial fuel cells.The ISME journal · 2012Article
- Interactive and Single Effects of Ectomycorrhiza Formation and Bacillus cereus on Metallothionein MT1 Expression and Phytoextraction of Cd and Zn by Willows.Water, air, and soil pollution · 2012Article
- Bromodeoxyuridine (BrdU) labeling and subsequent fluorescence activated cell sorting for culture-independent identification of dissolved organic carbon-degrading bacterioplankton.Journal of visualized experiments : JoVE · 2011Article
- Differential growth responses of soil bacterial taxa to carbon substrates of varying chemical recalcitrance.Frontiers in microbiology · 2011Article
- Microscopic detection of viable Staphylococcus epidermidis in peri-implant tissue in experimental biomaterial-associated infection, identified by bromodeoxyuridine incorporation.Infection and immunity · 2010Article
- Growth of enterococci in unaltered, unseeded beach sands subjected to tidal wetting.Applied and environmental microbiology · 2009Article
- Proper sanitization of sewage sludge: a critical issue for a sustainable society.Applied and environmental microbiology · 2008Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arbuscular mycorrhizae are beneficial for crops grown under low-till management systems. Increasingly, it is becoming apparent that bacteria associated with mycorrhizae can enhance the beneficial relationship between mycorrhizae and plants. However, it has been difficult to study these relationships by conventional techniques. In this study actively growing bacteria were identified in soil from an undisturbed fallow field known to contain arbuscular mycorrhizae by using molecular tools to eliminate the need for cultivation. A thymidine analog, bromodeoxyuridine (BrdU), was added to the soil and incubated for 2 days. DNA was extracted, and the newly synthesized DNA was isolated by immunocapture of the BrdU-containing DNA. The active bacteria in the community were identified by 16S rRNA gene PCR amplification and DNA sequence analysis. Based on 16S rRNA gene sequence information, a selective medium was chosen to isolate the corresponding active bacteria. Bacillus cereus strain VA1, one of the bacteria identified by the BrdU method, was isolated from the soil and tagged with green fluorescent protein. By using confocal microscopy, this bacterium was shown to clearly attach to arbuscular mycorrhizal hyphae. This study was the first to use this combination of molecular and traditional approaches to isolate, identify, and visualize a specific bacterium that is active in fallow soil and associates with arbuscular mycorrhizal hyphae.
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