ArticleApplied and environmental microbiology2003
Molecular analysis of the rfb O antigen gene cluster of Salmonella enterica serogroup O:6,14 and development of a serogroup-specific PCR assay.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 63 citations in OpenAlex.
- Genomic Characterization of an O-Antigen-Deficient, Hydrogen Sulfide-NegativeMicroorganisms · 2026Article
- The rfbA Gene as a High-Resolution Marker for Pathovar-Level Classification of Xanthomonas citri and Pseudomonas syringae.The plant pathology journal · 2026Article
- Multi-biome analysis identifies distinct gut microbial signatures and their crosstalk in ulcerative colitis and Crohn's disease.Nature communications · 2024Article
- Engineering a Novel Bivalent Oral Vaccine against Enteric Fever.International journal of molecular sciences · 2021Article
- Multiplex ligation reaction based on probe melting curve analysis: a pragmatic approach for the identification of 30 common Salmonella serovars.Annals of clinical microbiology and antimicrobials · 2019Article
- Prevalence and molecular characterization of Salmonella enterica serovar Typhimurium from ice and beverages in Jakarta, Indonesia.BMC research notes · 2019Article
- Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection ofMaterials (Basel, Switzerland) · 2018Article
- Designing a biochip following multiplex polymerase chain reaction for the detection of Salmonella serovars Typhimurium, Enteritidis, Infantis, Hadar, and Virchow in poultry products.Journal of food and drug analysis · 2018Article
- Evaluation of a multiplex PCR method to serotypeMethodsX · 2017Article
- Salmonella serotype determination utilizing high-throughput genome sequencing data.Journal of clinical microbiology · 2015Article
- Development of a DNA microarray for molecular identification of all 46 Salmonella O serogroups.Applied and environmental microbiology · 2013Article
- Comparison of typing methods with a new procedure based on sequence characterization for Salmonella serovar prediction.Journal of clinical microbiology · 2013Article
- Rapid genoserotyping tool for classification of Salmonella serovars.Journal of clinical microbiology · 2011Article
- Comprehensive approaches to molecular biomarker discovery for detection and identification of Cronobacter spp. (Enterobacter sakazakii) and Salmonella spp.Applied and environmental microbiology · 2011Article
- Development of O-antigen gene cluster-specific PCRs for rapid typing six epidemic serogroups of Leptospira in China.BMC microbiology · 2010Article
- Development and evaluation of a multiplex real-time polymerase chain reaction procedure to clinically type prevalent Salmonella enterica serovars.The Journal of molecular diagnostics : JMD · 2010Article
- Explaining microbial population genomics through phage predation.Nature reviews. Microbiology · 2009Review
- Salmonella serovar identification using PCR-based detection of gene presence and absence.Journal of clinical microbiology · 2008Article
- Multiplex, bead-based suspension array for molecular determination of common Salmonella serogroups.Journal of clinical microbiology · 2007Article
- Sequence analysis of the rfb loci, encoding proteins involved in the biosynthesis of the Salmonella enterica O17 and O18 antigens: serogroup-specific identification by PCR.Applied and environmental microbiology · 2006Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Kauffmann-White scheme for serotyping Salmonella recognizes 46 somatic (O) antigen groups, which together with detection of the flagellar (H) antigens form the basis for serotype identification. Although serotyping has become an invaluable typing method for epidemiological investigations of Salmonella, it does have some practical limitations. We have been characterizing the genes required for O and H antigen biosynthesis with the goal of developing a DNA-based system for the determination of serotype in Salmonella. The majority of the enzymes involved in O antigen biosynthesis are encoded by the rfb gene cluster. We report the sequencing of the rfb region from S. enterica serotype Sundsvall (serogroup O:6,14). The S. enterica serotype Sundsvall rfb region is 8.4 kb in length and comprises six open reading frames. When compared with other previously characterized rfb regions, the serogroup O:6,14 sequence is most related to serogroup C(1). On the basis of DNA sequence similarity, we identified two genes from the mannose biosynthetic pathway, two mannosyl transferase genes, the O unit flippase gene and, possibly, the O antigen polymerase. The whole cluster is derived from a low-G+C-content organism. Comparative sequencing of an additional serogroup O:6,14 isolate (S. enterica serotype Carrau) revealed a highly homologous sequence, suggesting that O antigen factors O:24 and O:25 (additional O factors associated with serogroup O:6,14) are encoded outside the rfb gene cluster. We developed a serogroup O:6,14-specific PCR assay based on a region of the putative wzx (O antigen flippase) gene. This provides the basis for a sensitive and specific test for the rapid identification of Salmonella serogroup O:6,14.
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