ArticleApplied and environmental microbiology2003
Peptide surface display and secretion using two LPXTG-containing surface proteins from Lactobacillus fermentum BR11.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
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- Putative Adhesion Factors in Vaginal Lactobacillus gasseri DSM 14869: Functional Characterization.Applied and environmental microbiology · 2019Article
- Display of a β-mannanase and a chitosanase on the cell surface of Lactobacillus plantarum towards the development of whole-cell biocatalysts.Microbial cell factories · 2016Article
- Formation of In Vitro Mixed-Species Biofilms by Lactobacillus pentosus and Yeasts Isolated from Spanish-Style Green Table Olive Fermentations.Applied and environmental microbiology · 2016Article
- Saccharomyces cerevisiae transcriptional reprograming due to bacterial contamination during industrial scale bioethanol production.Microbial cell factories · 2015Article
- High quality draft genome of Lactobacillus kunkeei EFB6, isolated from a German European foulbrood outbreak of honeybees.Standards in genomic sciences · 2015Article
- Effect of growth at low pH on the cell surface properties of a typical strain of Lactobacillus casei group.Iranian journal of microbiology · 2010Article
- Cystathionine gamma-lyase is a component of cystine-mediated oxidative defense in Lactobacillus reuteri BR11.Journal of bacteriology · 2009Article
- Random mutagenesis identifies novel genes involved in the secretion of antimicrobial, cell wall-lytic enzymes by Lactococcus lactis.Applied and environmental microbiology · 2008Article
- Inhibition of Staphylococcus aureus growth on tellurite-containing media by Lactobacillus reuteri Is dependent on CyuC and thiol production.Applied and environmental microbiology · 2007Article
- Display of alpha-amylase on the surface of Lactobacillus casei cells by use of the PgsA anchor protein, and production of lactic acid from starch.Applied and environmental microbiology · 2006Article
- Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM.Applied and environmental microbiology · 2005Article
- Biodiversity-based identification and functional characterization of the mannose-specific adhesin of Lactobacillus plantarum.Journal of bacteriology · 2005Article
- A high-molecular-mass surface protein (Lsp) and methionine sulfoxide reductase B (MsrB) contribute to the ecological performance of Lactobacillus reuteri in the murine gut.Applied and environmental microbiology · 2005Article
- Surface proteins of Streptococcus agalactiae and related proteins in other bacterial pathogens.Clinical microbiology reviews · 2005Review
- Identification and characterization of the novel LysM domain-containing surface protein Sep from Lactobacillus fermentum BR11 and its use as a peptide fusion partner in Lactobacillus and Lactococcus.Applied and environmental microbiology · 2004Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A locus encoding two repetitive proteins that have LPXTG cell wall anchoring signals from Lactobacillus fermentum BR11 has been identified by using an antiserum raised against whole L. fermentum BR11 cells. The first protein, Rlp, is similar to the Rib surface protein from Streptococcus agalactiae, while the other protein, Mlp, is similar to the mucus binding protein Mub from Lactobacillus reuteri. It was shown that multiple copies of mlp exist in the genome of L. fermentum BR11. Regions of Rlp, Mlp, and the previously characterized surface protein BspA were used to surface display or secrete heterologous peptides in L. fermentum. The peptides tested were 10 amino acids of the human cystic fibrosis transmembrane regulator protein and a six-histidine epitope (His(6)). The BspA promoter and secretion signal were used in combination with the Rlp cell wall sorting signal to express, export, and covalently anchor the heterologous peptides to the cell wall. Detection of the cell surface protein fusions revealed that Rlp was a significantly better surface display vector than BspA despite having lower cellular levels (0.7 mg per liter for the Rlp fusion compared with 4 mg per liter for the BspA fusion). The mlp promoter and encoded secretion signal were used to express and export large (328-kDa at 10 mg per liter) and small (27-kDa at 0.06 mg per liter) amino-terminal fragments of the Mlp protein fused to the His(6) and CFTR peptides or His(6) peptide, respectively. Therefore, these newly described proteins from L. fermentum BR11 have potential as protein production and targeting vectors.
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