ArticleNucleic acids research2003
Sequence-specific fluorescent labeling of double-stranded DNA observed at the single molecule level.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Review
- Applications of triplex DNA nanostructures in sensor development.Analytical and bioanalytical chemistry · 2022Review
- Advances in optical mapping for genomic research.Computational and structural biotechnology journal · 2020Review
- Novel glass slide preparation system for single DNA molecules analysis.Biotechnology, biotechnological equipment · 2014Article
- Is photocleavage of DNA by YOYO-1 using a synchrotron radiation light source sequence dependent?European biophysics journal : EBJ · 2011Article
- Labeling DNA for single-molecule experiments: methods of labeling internal specific sequences on double-stranded DNA.Nanoscale · 2011Review
- Peptide nucleic acids as tools for single-molecule sequence detection and manipulation.Nano letters · 2010Article
- Using triplex-forming oligonucleotide probes for the reagentless, electrochemical detection of double-stranded DNA.Analytical chemistry · 2010Article
- Single-molecule sequence detection via microfluidic planar extensional flow at a stagnation point.Lab on a chip · 2010Article
- Fluorescent marker for direct detection of specific dsDNA sequences.Analytical chemistry · 2009Article
- Novel sequencing strategy for repetitive DNA in a Drosophila BAC clone reveals that the centromeric region of the Y chromosome evolved from a telomere.Nucleic acids research · 2009Article
- The triple helix: 50 years later, the outcome.Nucleic acids research · 2008Review
- Labeling of unique sequences in double-stranded DNA at sites of vicinal nicks generated by nicking endonucleases.Nucleic acids research · 2008Article
- Detection of single DNA molecules by multicolor quantum-dot end-labeling.Nucleic acids research · 2005Article
- Marking of specific sequences in double-stranded DNA molecules--SNP detection and direct observation.Genome research · 2004Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent labeling of a short sequence of double-stranded DNA (dsDNA) was achieved by ligating a labeled dsDNA fragment to a stem-loop triplex forming oligonucleotide (TFO). After the TFO has wound around the target sequence by ligand-induced triple helix formation, its extremities hybridize to each other, leaving a dangling single-stranded sequence, which is then ligated to a fluorescent dsDNA fragment using T4 DNA ligase. A non-repeated 15 bp sequence present on lambda DNA was labeled and visualized by fluorescence microscopy after DNA combing. The label was found to be attached at a specific position located at 4.2 +/- 0.5 kb from one end of the molecule, in agreement with the location of the target sequence for triple helix formation (4.4 kb from one end). In addition, an alternative combing process was noticed in which a DNA molecule becomes attached to the combing slide from the label rather than from one of its ends. The method described herein provides a new tool for the detection of very short sequences of dsDNA and offers various perspectives in the micromanipulation of single DNA molecules.
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