ArticleThe Journal of cell biology2003
Ligand crowding at a nascent signal sequence.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 65 citations in OpenAlex.
- An intrinsically disordered nascent protein interacts with specific regions of the ribosomal surface near the exit tunnel.Communications biology · 2021Article
- The Dynamic SecYEG Translocon.Frontiers in molecular biosciences · 2021Review
- How Quality Control Systems AID Sec-Dependent Protein Translocation.Frontiers in molecular biosciences · 2021Review
- Article
- The bacterial protein YidC accelerates MPIase-dependent integration of membrane proteins.The Journal of biological chemistry · 2019Article
- Molecular Mimicry of SecA and Signal Recognition Particle Binding to the Bacterial Ribosome.mBio · 2019Article
- Crosslinking and Reconstitution Approaches to Study Protein Transport.The protein journal · 2019Review
- The way is the goal: how SecA transports proteins across the cytoplasmic membrane in bacteria.FEMS microbiology letters · 2018Review
- SecA Cotranslationally Interacts with Nascent Substrate Proteins In Vivo.Journal of bacteriology · 2017Article
- Signal recognition particle and SecA cooperate during export of secretory proteins with highly hydrophobic signal sequences.PloS one · 2014Article
- Signal sequence-independent SRP-SR complex formation at the membrane suggests an alternative targeting pathway within the SRP cycle.Molecular biology of the cell · 2011Article
- TatB functions as an oligomeric binding site for folded Tat precursor proteins.Molecular biology of the cell · 2010Article
- The conformation of a nascent polypeptide inside the ribosome tunnel affects protein targeting and protein folding.Molecular microbiology · 2010Article
- Versatility of trigger factor interactions with ribosome-nascent chain complexes.The Journal of biological chemistry · 2010Article
- Predominant membrane localization is an essential feature of the bacterial signal recognition particle receptor.BMC biology · 2009Article
- Differential bacterial surface display of peptides by the transmembrane domain of OmpA.PloS one · 2009Article
- The ribosome as a platform for co-translational processing, folding and targeting of newly synthesized proteins.Nature structural & molecular biology · 2009Review
- Delivering proteins for export from the cytosol.Nature reviews. Molecular cell biology · 2009Review
- Mapping the pathways to staphylococcal pathogenesis by comparative secretomics.Microbiology and molecular biology reviews : MMBR · 2006Review
- L25 functions as a conserved ribosomal docking site shared by nascent chain-associated complex and signal-recognition particle.EMBO reports · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have systematically analyzed the molecular environment of the signal sequence of a growing secretory protein from Escherichia coli using a stage- and site-specific cross-linking approach. Immediately after emerging from the ribosome, the signal sequence of pOmpA is accessible to Ffh, the protein component of the bacterial signal recognition particle, and to SecA, but it remains attached to the surface of the ribosome via protein L23. These contacts are lost upon further growth of the nascent chain, which brings the signal sequence into sole proximity to the chaperone Trigger factor (TF). In its absence, nascent pOmpA shows extended contacts with L23, and even long chains interact in these conditions proficiently with Ffh. Our results suggest that upon emergence from the ribosome, the signal sequence of an E. coli secretory protein gradually becomes sequestered by TF. Although TF thereby might control the accessibility of pOmpA's signal sequence to Ffh and SecA, it does not influence interaction of pOmpA with SecB.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.